Description
The mRNA track shows alignments between S. cerevisiae mRNAs
in GenBank and the genome.
Display Conventions and Configuration
This track follows the display conventions for
PSL alignment tracks. In dense display mode, the items that
are more darkly shaded indicate matches of better quality.
The description page for this track has a filter that can be used to change
the display mode, alter the color, and include/exclude a subset of items
within the track. This may be helpful when many items are shown in the track
display, especially when only some are relevant to the current task.
To use the filter:
- Type a term in one or more of the text boxes to filter the mRNA
display. For example, to apply the filter to all mRNAs submitted by a specific
author, type the name of the individual in the author box. To view the list of
valid terms for each text box, consult the table in the Table Browser that
corresponds to the factor on which you wish to filter. For example, the
"author" table contains the names of all individuals who can be
entered into the author text box. Wildcards may also be used in the
filter.
- If filtering on more than one value, choose the desired combination
logic. If "and" is selected, only mRNAs that match all filter
criteria will be displayed. If "or" is selected, only mRNAs that
match any one of the filter criteria will be displayed.
- Choose the color or display characteristic that should be used to
highlight or include/exclude the filtered items. If "exclude" is
chosen, the browser will not display mRNAs that match the filter criteria.
If "include" is selected, the browser will display only those
mRNAs that match the filter criteria.
This track may also be configured to display codon coloring, a feature that
allows the user to quickly compare mRNAs against the genomic sequence. For more
information about this option, click
here.
Methods
GenBank S. cerevisiae mRNAs were aligned against the genome using the
blat program. When a single mRNA aligned in multiple places,
the alignment having the highest base identity was found.
Only alignments having a base identity level within 0.5% of
the best and at least 96% base identity with the genomic sequence were kept.
Credits
The mRNA track was produced at UCSC from mRNA sequence data
submitted to the international public sequence databases by
scientists worldwide.
References
Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL.
GenBank: update.
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6.
PMID: 14681350; PMC: PMC308779
Kent WJ.
BLAT - the BLAST-like alignment tool.
Genome Res. 2002 Apr;12(4):656-64.
PMID: 11932250; PMC: PMC187518
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