Mouse methylome studies SRP312491 Track Settings
 
Placenta and fetal brain share a neurodevelopmental disorder DNA methylation profile in a mouse model of prenatal PCB exposure [WGBS] [Brain, Placenta]

Track collection: Mouse methylome studies

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experiment
SRX10463318 
SRX10463319 
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SRX10463321 
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SRX10463404 
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experiment
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 SRX10463318  HMR  Brain / SRX10463318 (HMR)   Data format 
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 SRX10463318  CpG methylation  Brain / SRX10463318 (CpG methylation)   Data format 
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 SRX10463319  HMR  Brain / SRX10463319 (HMR)   Data format 
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 SRX10463319  CpG methylation  Brain / SRX10463319 (CpG methylation)   Data format 
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 SRX10463320  HMR  Brain / SRX10463320 (HMR)   Data format 
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 SRX10463320  CpG methylation  Brain / SRX10463320 (CpG methylation)   Data format 
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 SRX10463321  HMR  Brain / SRX10463321 (HMR)   Data format 
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 SRX10463321  CpG methylation  Brain / SRX10463321 (CpG methylation)   Data format 
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 SRX10463322  HMR  Brain / SRX10463322 (HMR)   Data format 
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 SRX10463322  CpG methylation  Brain / SRX10463322 (CpG methylation)   Data format 
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 SRX10463323  HMR  Brain / SRX10463323 (HMR)   Data format 
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 SRX10463323  CpG methylation  Brain / SRX10463323 (CpG methylation)   Data format 
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 SRX10463324  HMR  Brain / SRX10463324 (HMR)   Data format 
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 SRX10463324  CpG methylation  Brain / SRX10463324 (CpG methylation)   Data format 
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 SRX10463325  HMR  Brain / SRX10463325 (HMR)   Data format 
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 SRX10463325  CpG methylation  Brain / SRX10463325 (CpG methylation)   Data format 
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 SRX10463326  HMR  Brain / SRX10463326 (HMR)   Data format 
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 SRX10463326  CpG methylation  Brain / SRX10463326 (CpG methylation)   Data format 
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 SRX10463327  HMR  Brain / SRX10463327 (HMR)   Data format 
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 SRX10463327  CpG methylation  Brain / SRX10463327 (CpG methylation)   Data format 
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 SRX10463328  HMR  Brain / SRX10463328 (HMR)   Data format 
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 SRX10463328  CpG methylation  Brain / SRX10463328 (CpG methylation)   Data format 
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 SRX10463329  HMR  Brain / SRX10463329 (HMR)   Data format 
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 SRX10463329  CpG methylation  Brain / SRX10463329 (CpG methylation)   Data format 
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 SRX10463330  HMR  Brain / SRX10463330 (HMR)   Data format 
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 SRX10463330  CpG methylation  Brain / SRX10463330 (CpG methylation)   Data format 
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 SRX10463331  HMR  Brain / SRX10463331 (HMR)   Data format 
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 SRX10463331  CpG methylation  Brain / SRX10463331 (CpG methylation)   Data format 
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 SRX10463332  HMR  Brain / SRX10463332 (HMR)   Data format 
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 SRX10463332  CpG methylation  Brain / SRX10463332 (CpG methylation)   Data format 
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 SRX10463333  HMR  Brain / SRX10463333 (HMR)   Data format 
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 SRX10463333  CpG methylation  Brain / SRX10463333 (CpG methylation)   Data format 
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 SRX10463334  HMR  Brain / SRX10463334 (HMR)   Data format 
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 SRX10463334  CpG methylation  Brain / SRX10463334 (CpG methylation)   Data format 
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 SRX10463335  HMR  Brain / SRX10463335 (HMR)   Data format 
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 SRX10463335  CpG methylation  Brain / SRX10463335 (CpG methylation)   Data format 
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 SRX10463336  HMR  Brain / SRX10463336 (HMR)   Data format 
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 SRX10463336  CpG methylation  Brain / SRX10463336 (CpG methylation)   Data format 
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 SRX10463337  HMR  Brain / SRX10463337 (HMR)   Data format 
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 SRX10463337  CpG methylation  Brain / SRX10463337 (CpG methylation)   Data format 
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 SRX10463338  HMR  Brain / SRX10463338 (HMR)   Data format 
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 SRX10463338  CpG methylation  Brain / SRX10463338 (CpG methylation)   Data format 
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 SRX10463339  HMR  Brain / SRX10463339 (HMR)   Data format 
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 SRX10463339  CpG methylation  Brain / SRX10463339 (CpG methylation)   Data format 
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 SRX10463340  HMR  Brain / SRX10463340 (HMR)   Data format 
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 SRX10463340  CpG methylation  Brain / SRX10463340 (CpG methylation)   Data format 
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 SRX10463341  HMR  Brain / SRX10463341 (HMR)   Data format 
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 SRX10463341  CpG methylation  Brain / SRX10463341 (CpG methylation)   Data format 
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 SRX10463342  HMR  Brain / SRX10463342 (HMR)   Data format 
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 SRX10463342  CpG methylation  Brain / SRX10463342 (CpG methylation)   Data format 
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 SRX10463343  HMR  Brain / SRX10463343 (HMR)   Data format 
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 SRX10463343  CpG methylation  Brain / SRX10463343 (CpG methylation)   Data format 
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 SRX10463344  HMR  Brain / SRX10463344 (HMR)   Data format 
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 SRX10463344  CpG methylation  Brain / SRX10463344 (CpG methylation)   Data format 
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 SRX10463345  HMR  Brain / SRX10463345 (HMR)   Data format 
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 SRX10463345  CpG methylation  Brain / SRX10463345 (CpG methylation)   Data format 
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 SRX10463346  HMR  Brain / SRX10463346 (HMR)   Data format 
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 SRX10463346  CpG methylation  Brain / SRX10463346 (CpG methylation)   Data format 
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 SRX10463347  HMR  Brain / SRX10463347 (HMR)   Data format 
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 SRX10463347  CpG methylation  Brain / SRX10463347 (CpG methylation)   Data format 
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 SRX10463348  HMR  Brain / SRX10463348 (HMR)   Data format 
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 SRX10463348  CpG methylation  Brain / SRX10463348 (CpG methylation)   Data format 
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 SRX10463349  HMR  Brain / SRX10463349 (HMR)   Data format 
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 SRX10463349  CpG methylation  Brain / SRX10463349 (CpG methylation)   Data format 
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 SRX10463350  HMR  Brain / SRX10463350 (HMR)   Data format 
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 SRX10463350  CpG methylation  Brain / SRX10463350 (CpG methylation)   Data format 
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 SRX10463351  HMR  Brain / SRX10463351 (HMR)   Data format 
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 SRX10463351  CpG methylation  Brain / SRX10463351 (CpG methylation)   Data format 
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 SRX10463352  HMR  Brain / SRX10463352 (HMR)   Data format 
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 SRX10463352  CpG methylation  Brain / SRX10463352 (CpG methylation)   Data format 
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 SRX10463353  HMR  Brain / SRX10463353 (HMR)   Data format 
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 SRX10463353  CpG methylation  Brain / SRX10463353 (CpG methylation)   Data format 
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 SRX10463354  HMR  Brain / SRX10463354 (HMR)   Data format 
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 SRX10463354  CpG methylation  Brain / SRX10463354 (CpG methylation)   Data format 
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 SRX10463355  HMR  Brain / SRX10463355 (HMR)   Data format 
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 SRX10463355  CpG methylation  Brain / SRX10463355 (CpG methylation)   Data format 
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 SRX10463356  HMR  Brain / SRX10463356 (HMR)   Data format 
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 SRX10463356  CpG methylation  Brain / SRX10463356 (CpG methylation)   Data format 
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 SRX10463357  HMR  Brain / SRX10463357 (HMR)   Data format 
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 SRX10463357  CpG methylation  Brain / SRX10463357 (CpG methylation)   Data format 
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 SRX10463358  HMR  Brain / SRX10463358 (HMR)   Data format 
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 SRX10463358  CpG methylation  Brain / SRX10463358 (CpG methylation)   Data format 
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 SRX10463359  HMR  Brain / SRX10463359 (HMR)   Data format 
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 SRX10463359  CpG methylation  Brain / SRX10463359 (CpG methylation)   Data format 
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 SRX10463360  HMR  Brain / SRX10463360 (HMR)   Data format 
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 SRX10463360  CpG methylation  Brain / SRX10463360 (CpG methylation)   Data format 
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 SRX10463361  HMR  Brain / SRX10463361 (HMR)   Data format 
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 SRX10463361  CpG methylation  Brain / SRX10463361 (CpG methylation)   Data format 
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 SRX10463362  CpG methylation  Placenta / SRX10463362 (CpG methylation)   Data format 
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 SRX10463363  CpG methylation  Placenta / SRX10463363 (CpG methylation)   Data format 
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 SRX10463364  CpG methylation  Placenta / SRX10463364 (CpG methylation)   Data format 
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 SRX10463365  CpG methylation  Placenta / SRX10463365 (CpG methylation)   Data format 
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 SRX10463366  CpG methylation  Placenta / SRX10463366 (CpG methylation)   Data format 
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 SRX10463367  CpG methylation  Placenta / SRX10463367 (CpG methylation)   Data format 
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 SRX10463368  CpG methylation  Placenta / SRX10463368 (CpG methylation)   Data format 
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 SRX10463369  CpG methylation  Placenta / SRX10463369 (CpG methylation)   Data format 
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 SRX10463370  CpG methylation  Placenta / SRX10463370 (CpG methylation)   Data format 
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 SRX10463371  CpG methylation  Placenta / SRX10463371 (CpG methylation)   Data format 
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 SRX10463372  CpG methylation  Placenta / SRX10463372 (CpG methylation)   Data format 
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 SRX10463373  CpG methylation  Placenta / SRX10463373 (CpG methylation)   Data format 
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 SRX10463374  CpG methylation  Placenta / SRX10463374 (CpG methylation)   Data format 
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 SRX10463375  CpG methylation  Placenta / SRX10463375 (CpG methylation)   Data format 
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 SRX10463376  CpG methylation  Placenta / SRX10463376 (CpG methylation)   Data format 
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 SRX10463377  CpG methylation  Placenta / SRX10463377 (CpG methylation)   Data format 
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 SRX10463378  CpG methylation  Placenta / SRX10463378 (CpG methylation)   Data format 
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 SRX10463379  CpG methylation  Placenta / SRX10463379 (CpG methylation)   Data format 
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 SRX10463380  CpG methylation  Placenta / SRX10463380 (CpG methylation)   Data format 
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 SRX10463381  CpG methylation  Placenta / SRX10463381 (CpG methylation)   Data format 
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 SRX10463382  CpG methylation  Placenta / SRX10463382 (CpG methylation)   Data format 
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 SRX10463383  CpG methylation  Placenta / SRX10463383 (CpG methylation)   Data format 
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 SRX10463384  CpG methylation  Placenta / SRX10463384 (CpG methylation)   Data format 
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 SRX10463385  CpG methylation  Placenta / SRX10463385 (CpG methylation)   Data format 
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 SRX10463386  CpG methylation  Placenta / SRX10463386 (CpG methylation)   Data format 
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 SRX10463387  CpG methylation  Placenta / SRX10463387 (CpG methylation)   Data format 
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 SRX10463388  CpG methylation  Placenta / SRX10463388 (CpG methylation)   Data format 
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 SRX10463389  CpG methylation  Placenta / SRX10463389 (CpG methylation)   Data format 
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 SRX10463390  CpG methylation  Placenta / SRX10463390 (CpG methylation)   Data format 
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 SRX10463391  CpG methylation  Placenta / SRX10463391 (CpG methylation)   Data format 
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 SRX10463392  CpG methylation  Placenta / SRX10463392 (CpG methylation)   Data format 
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 SRX10463393  CpG methylation  Placenta / SRX10463393 (CpG methylation)   Data format 
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 SRX10463394  CpG methylation  Placenta / SRX10463394 (CpG methylation)   Data format 
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 SRX10463395  CpG methylation  Placenta / SRX10463395 (CpG methylation)   Data format 
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 SRX10463396  CpG methylation  Placenta / SRX10463396 (CpG methylation)   Data format 
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 SRX10463397  CpG methylation  Placenta / SRX10463397 (CpG methylation)   Data format 
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 SRX10463398  CpG methylation  Placenta / SRX10463398 (CpG methylation)   Data format 
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 SRX10463399  CpG methylation  Placenta / SRX10463399 (CpG methylation)   Data format 
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 SRX10463400  CpG methylation  Placenta / SRX10463400 (CpG methylation)   Data format 
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 SRX10463401  CpG methylation  Placenta / SRX10463401 (CpG methylation)   Data format 
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 SRX10463402  CpG methylation  Placenta / SRX10463402 (CpG methylation)   Data format 
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 SRX10463403  CpG methylation  Placenta / SRX10463403 (CpG methylation)   Data format 
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 SRX10463404  CpG methylation  Placenta / SRX10463404 (CpG methylation)   Data format 
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 SRX10463405  CpG methylation  Placenta / SRX10463405 (CpG methylation)   Data format 
    
Assembly: Mouse Jun. 2020 (GRCm39/mm39)

Study title: Placenta and fetal brain share a neurodevelopmental disorder DNA methylation profile in a mouse model of prenatal PCB exposure [WGBS]
SRA: SRP312491
GEO: GSE171016
Pubmed: 35235788

Experiment Label Methylation Coverage HMRs HMR size AMRs AMR size PMDs PMD size Conversion Title
SRX10463318 Brain 0.760 6.1 45898 1338.5 162 1107.7 940 21244.1 0.986 GSM5216345: D101-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463319 Brain 0.754 7.1 48888 1270.1 197 1052.3 1246 17660.7 0.985 GSM5216346: D102-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463320 Brain 0.760 6.6 49402 1288.6 162 1047.8 1211 19202.0 0.986 GSM5216347: D103-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463321 Brain 0.756 8.1 51597 1235.0 242 1011.6 1612 13886.0 0.986 GSM5216348: D104-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463322 Brain 0.757 6.9 49857 1274.2 241 1072.5 966 21099.0 0.986 GSM5216349: D201-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463323 Brain 0.760 6.4 46959 1324.1 206 1134.4 984 20654.9 0.983 GSM5216350: D203-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463324 Brain 0.759 5.4 45223 1370.3 156 1114.5 1141 19305.8 0.983 GSM5216351: D206-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463325 Brain 0.758 6.1 47081 1328.4 146 1202.6 1135 18513.6 0.983 GSM5216352: D207-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463326 Brain 0.760 7.2 49650 1268.8 269 1084.2 1275 19386.7 0.983 GSM5216353: B201-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463327 Brain 0.761 5.9 47363 1329.2 135 1155.2 1297 17176.0 0.983 GSM5216354: B202-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463328 Brain 0.765 5.9 47292 1332.4 115 1207.7 1114 18246.5 0.983 GSM5216355: B203-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463329 Brain 0.762 6.7 48941 1298.4 231 1093.3 1120 20034.8 0.983 GSM5216356: B204-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463330 Brain 0.762 7.0 49379 1272.9 203 1049.9 1274 19150.0 0.983 GSM5216357: F102-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463331 Brain 0.763 6.4 47765 1310.7 145 1110.3 1410 18269.5 0.983 GSM5216358: F103-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463332 Brain 0.761 7.0 49454 1276.6 211 1116.5 915 21680.0 0.983 GSM5216359: F104-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463333 Brain 0.759 6.8 49567 1274.8 201 1048.3 1176 19786.0 0.983 GSM5216360: F105-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463334 Brain 0.768 6.3 48377 1315.0 151 1095.5 1171 19628.1 0.983 GSM5216361: F106-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463335 Brain 0.770 6.7 48469 1304.0 149 1110.4 1255 19374.9 0.983 GSM5216362: F107-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463336 Brain 0.766 5.9 47413 1327.9 110 1200.4 1280 18957.5 0.983 GSM5216363: F108-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463337 Brain 0.757 6.4 49552 1308.0 195 1081.2 1141 19911.6 0.983 GSM5216364: C201-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463338 Brain 0.754 6.5 48881 1299.9 186 1074.3 1144 19684.6 0.983 GSM5216365: C202-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463339 Brain 0.757 6.6 48514 1293.6 195 1110.7 1021 20230.0 0.983 GSM5216366: C203-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463340 Brain 0.759 7.7 52350 1253.8 270 1112.7 1936 12953.8 0.983 GSM5216367: C204-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463341 Brain 0.752 6.1 49085 1301.3 225 1041.4 1146 19219.4 0.983 GSM5216368: C205-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463342 Brain 0.755 6.2 48706 1301.4 185 1071.5 1147 19574.8 0.983 GSM5216369: C206-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463343 Brain 0.756 7.2 51322 1265.2 217 1131.0 1063 20543.5 0.983 GSM5216370: C207-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463344 Brain 0.752 6.8 50751 1267.4 209 1047.7 1242 17443.8 0.983 GSM5216371: F201-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463345 Brain 0.754 6.8 51048 1266.5 194 1030.5 1146 18144.7 0.983 GSM5216372: F202-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463346 Brain 0.753 6.7 50200 1280.0 190 1149.5 1405 18366.3 0.983 GSM5216373: F203-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463347 Brain 0.754 6.8 49673 1276.7 208 1069.4 1252 17624.3 0.983 GSM5216374: F207-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463348 Brain 0.757 7.7 52036 1238.7 233 1075.3 1221 19331.9 0.983 GSM5216375: F208-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463349 Brain 0.754 7.3 50732 1267.6 267 1067.5 1190 19554.2 0.983 GSM5216376: E205-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463350 Brain 0.753 6.0 47813 1327.8 140 1106.9 1052 18784.5 0.983 GSM5216377: E206-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463351 Brain 0.755 7.0 50176 1277.9 247 1076.6 1083 19809.5 0.983 GSM5216378: E207-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463352 Brain 0.751 6.7 50106 1287.5 188 1060.9 1131 17923.7 0.983 GSM5216379: A101-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463353 Brain 0.750 6.8 50651 1286.0 242 1047.3 1116 20088.2 0.982 GSM5216380: A102-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463354 Brain 0.755 5.7 46543 1351.9 131 1088.9 1193 19192.9 0.983 GSM5216381: A103-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463355 Brain 0.757 6.8 50306 1296.7 206 1039.2 1090 19956.3 0.983 GSM5216382: A104-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463356 Brain 0.758 7.0 50247 1257.4 182 1090.2 1051 20293.4 0.984 GSM5216383: B102-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463357 Brain 0.757 6.1 47460 1311.2 136 1146.7 867 21894.1 0.983 GSM5216384: B103-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463358 Brain 0.759 6.9 50107 1263.2 191 1056.6 1286 18611.2 0.983 GSM5216385: B104-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463359 Brain 0.759 5.9 46865 1329.9 137 1128.3 1254 18891.4 0.983 GSM5216386: B105-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463360 Brain 0.754 6.1 49079 1306.7 142 1124.0 1017 19085.8 0.983 GSM5216387: B107-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463361 Brain 0.753 6.2 47350 1312.3 211 1063.5 1217 18922.1 0.983 GSM5216388: B108-Brain (WGBS); Mus musculus; Bisulfite-Seq
SRX10463362 Placenta 0.520 6.9 32430 7732.5 137 896.2 1255 745073.5 0.982 GSM5216389: D101-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463363 Placenta 0.508 7.7 35027 8199.2 188 935.0 1117 838582.7 0.982 GSM5216390: D102-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463364 Placenta 0.511 7.0 32054 7763.2 133 938.5 1176 782368.0 0.982 GSM5216391: D103-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463365 Placenta 0.513 6.3 29501 7148.2 106 1065.5 1173 773745.0 0.982 GSM5216392: D104-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463366 Placenta 0.537 7.5 27675 3252.7 237 873.5 1131 791566.8 0.982 GSM5216393: D201-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463367 Placenta 0.530 4.9 26879 8430.7 54 1025.4 957 991966.3 0.982 GSM5216394: D203-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463368 Placenta 0.515 6.7 32542 8422.8 146 960.5 1256 752355.4 0.982 GSM5216395: D206-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463369 Placenta 0.509 6.6 33297 8925.8 126 947.9 1132 810600.7 0.982 GSM5216396: D207-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463370 Placenta 0.523 6.7 28892 5702.8 154 912.5 1116 854467.0 0.983 GSM5216397: B201-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463371 Placenta 0.520 6.5 27668 5598.3 151 920.6 1143 780169.1 0.982 GSM5216398: B202-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463372 Placenta 0.517 6.5 28579 5402.7 161 961.2 1122 771061.3 0.982 GSM5216399: B203-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463373 Placenta 0.534 6.3 26666 4007.6 161 938.3 1088 834776.1 0.982 GSM5216400: B204-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463374 Placenta 0.486 7.1 29693 11306.2 119 4627.9 1269 743829.3 0.982 GSM5216401: F102-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463375 Placenta 0.487 6.3 27464 10767.2 95 899.7 1100 843293.3 0.982 GSM5216402: F103-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463376 Placenta 0.501 6.6 31547 9109.7 139 910.5 1238 778400.0 0.982 GSM5216403: F104-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463377 Placenta 0.504 6.2 29639 8592.2 115 901.2 1217 784809.0 0.983 GSM5216404: F105-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463378 Placenta 0.496 6.8 30715 9484.9 145 868.2 1181 788954.6 0.982 GSM5216405: F106-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463379 Placenta 0.498 6.8 30907 8607.2 149 976.9 1171 804304.9 0.983 GSM5216406: F107-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463380 Placenta 0.478 7.0 28376 11042.5 118 937.8 1314 717235.3 0.982 GSM5216407: F108-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463381 Placenta 0.524 5.1 26083 8390.9 54 956.0 988 950817.4 0.982 GSM5216408: C201-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463382 Placenta 0.509 6.5 29886 9355.6 125 944.5 1259 748585.3 0.982 GSM5216409: C202-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463383 Placenta 0.526 6.1 29792 7166.7 112 1052.0 1177 796216.5 0.982 GSM5216410: C203-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463384 Placenta 0.509 5.1 23802 9333.5 131 926.4 961 980442.0 0.982 GSM5216411: C204-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463385 Placenta 0.523 5.9 28497 6949.1 134 892.2 1060 880144.2 0.982 GSM5216412: C205-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463386 Placenta 0.524 4.8 25638 7921.8 63 1053.7 911 1015993.5 0.983 GSM5216413: C206-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463387 Placenta 0.523 6.3 30757 7414.6 110 889.0 1157 805990.2 0.982 GSM5216414: C207-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463388 Placenta 0.522 5.9 28460 6604.3 110 1008.5 911 970004.3 0.982 GSM5216415: F201-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463389 Placenta 0.522 7.0 30604 5132.0 208 890.0 1190 756567.1 0.982 GSM5216416: F202-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463390 Placenta 0.507 5.9 27785 10257.6 82 952.2 1193 810839.7 0.982 GSM5216417: F203-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463391 Placenta 0.521 6.5 29657 6455.0 122 990.9 1127 807149.2 0.982 GSM5216418: F207-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463392 Placenta 0.515 6.4 29487 6524.2 134 988.3 1124 800566.7 0.983 GSM5216419: F208-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463393 Placenta 0.540 7.3 27395 3512.6 269 903.4 1144 795748.8 0.982 GSM5216420: E205-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463394 Placenta 0.524 6.1 28774 6632.2 119 917.5 876 1045626.2 0.983 GSM5216421: E206-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463395 Placenta 0.535 6.5 30975 6499.7 147 917.4 1135 819549.7 0.982 GSM5216422: E207-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463396 Placenta 0.513 5.8 29165 9178.5 91 1041.1 969 971561.6 0.982 GSM5216423: A101-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463397 Placenta 0.538 6.3 30663 5594.9 115 987.1 1123 809165.0 0.982 GSM5216424: A102-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463398 Placenta 0.525 6.4 30207 8255.4 118 912.6 1213 790102.8 0.982 GSM5216425: A103-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463399 Placenta 0.513 5.4 25231 10127.8 103 952.1 942 983052.4 0.981 GSM5216426: A104-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463400 Placenta 0.503 6.5 30148 8590.9 136 951.3 1102 818387.3 0.982 GSM5216427: B102-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463401 Placenta 0.520 6.7 30939 6020.6 178 894.0 1149 820297.2 0.983 GSM5216428: B103-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463402 Placenta 0.527 7.1 26659 3673.1 200 896.7 1115 776343.9 0.982 GSM5216429: B104-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463403 Placenta 0.519 6.7 29246 6130.7 170 926.8 1117 838911.2 0.982 GSM5216430: B105-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463404 Placenta 0.515 4.9 25962 8243.9 51 953.2 884 1010173.6 0.983 GSM5216431: B107-Placenta (WGBS); Mus musculus; Bisulfite-Seq
SRX10463405 Placenta 0.516 5.9 27843 8795.0 85 922.7 1164 802455.1 0.982 GSM5216432: B108-Placenta (WGBS); Mus musculus; Bisulfite-Seq

Methods

All analysis was done using a bisulfite sequnecing data analysis pipeline DNMTools developed in the Smith lab at USC.

Mapping reads from bisulfite sequencing: Bisulfite treated reads are mapped to the genomes with the abismal program. Input reads are filtered by their quality, and adapter sequences in the 3' end of reads are trimmed. This is done with cutadapt. Uniquely mapped reads with mismatches/indels below given threshold are retained. For pair-end reads, if the two mates overlap, the overlapping part of the mate with lower quality is discarded. After mapping, we use the format command in dnmtools to merge mates for paired-end reads. We use the dnmtools uniq command to randomly select one from multiple reads mapped exactly to the same location. Without random oligos as UMIs, this is our best indication of PCR duplicates.

Estimating methylation levels: After reads are mapped and filtered, the dnmtools counts command is used to obtain read coverage and estimate methylation levels at individual cytosine sites. We count the number of methylated reads (those containing a C) and the number of unmethylated reads (those containing a T) at each nucleotide in a mapped read that corresponds to a cytosine in the reference genome. The methylation level of that cytosine is estimated as the ratio of methylated to total reads covering that cytosine. For cytosines in the symmetric CpG sequence context, reads from the both strands are collapsed to give a single estimate. Very rarely do the levels differ between strands (typically only if there has been a substitution, as in a somatic mutation), and this approach gives a better estimate.

Bisulfite conversion rate: The bisulfite conversion rate for an experiment is estimated with the dnmtools bsrate command, which computes the fraction of successfully converted nucleotides in reads (those read out as Ts) among all nucleotides in the reads mapped that map over cytosines in the reference genome. This is done either using a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear genome. In the latter case, only non-CpG sites are used. While this latter approach can be impacted by non-CpG cytosine methylation, in practice it never amounts to much.

Identifying hypomethylated regions (HMRs): In most mammalian cells, the majority of the genome has high methylation, and regions of low methylation are typically the interesting features. (This seems to be true for essentially all healthy differentiated cell types, but not cells of very early embryogenesis, various germ cells and precursors, and placental lineage cells.) These are valleys of low methylation are called hypomethylated regions (HMR) for historical reasons. To identify the HMRs, we use the dnmtools hmr command, which uses a statistical model that accounts for both the methylation level fluctations and the varying amounts of data available at each CpG site.

Partially methylated domains: Partially methylated domains are large genomic regions showing partial methylation observed in immortalized cell lines and cancerous cells. The pmd program is used to identify PMDs.

Allele-specific methylation: Allele-Specific methylated regions refers to regions where the parental allele is differentially methylated compared to the maternal allele. The program allelic is used to compute allele-specific methylation score can be computed for each CpG site by testing the linkage between methylation status of adjacent reads, and the program amrfinder is used to identify regions with allele-specific methylation.

For more detailed description of the methods of each step, please refer to the DNMTools documentation.