Assembly: Ebola virus Sierra Leone 2014 (G3683/KM034562.1/eboVir3) Data last updated at UCSC: 2014-12-04 06:47:49
Description
This track shows peptides that are predicted to be displayed by
human class II MHCs.
Display Conventions and Configuration
Peptides are shaded by percentile.
Methods
For human epitope predictions, 15-mer peptides overlapping by 10 aa residues
were generated from aligned sequences, to avoid redundant peptides that share
the same 9-mer binding core. For each 15-mer peptide, the
binding affinity was predicted (expressed as the IEDB consensus percentile
rank) for seven class II human HLA alleles (HLA-DRB1*03:01, HLA-DRB1*07:01,
HLA-DRB1*15:01, HLA-DRB3*01:01, HLA-DRB3*02:02, HLADRB4*01:01 and
HLA-DRB5*01:01), using the MHC
class I binding prediction tool ("IEDB recommended" method). Predicted
binders were selected based on the median consensus percentile rank estimated from the
consensus percentile ranks for the seven alleles. All peptides with median
consensus percentile rank <= 20.0 were selected as predicted binders, the
threshold being previously optimized for capturing 50% of class II human T cell
responses.