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ADCYAP1 — MAPK15

Text-mined interactions from Literome

Sakai et al., Biochem Biophys Res Commun 2001 : We have recently shown that in PC12 cells, PACAP and NGF synergistically increase PACAP gene transcription and mRNA level, and that the MAPK/ERK kinase inhibitor PD98059 blocks the PACAP mRNA expression induced by either PACAP or NGF, but not that induced by the combination, suggesting involvement of multiple signaling pathways
Dziema et al., J Neurophysiol 2002 (Second Messenger Systems) : Immunocytochemistry and Western analysis confirmed that PACAP stimulates MAPK activity at doses and time points shown to potentiate Ca ( 2+ ) influx
Moody et al., Regul Pept 2002 (Lung Neoplasms) : PACAP-27 ( 100 nM ) increased MAPK tyrosine phosphorylation 3-fold, 5 min after addition to NCI-H1299 cells ... PACAP-27 or PACAP-38 ( 100 nM ) but not PACAP28-38 or VIP caused increased MAPK tyrosine phosphorylation using NCI-H1299 cells ... Also, the increase in MAPK tyrosine phosphorylation caused by PACAP-27 was totally inhibited by 10 microM PACAP ( 6-38 ), a PAC(1) receptor antagonist or 10 microM PD98059, a MAPKK inhibitor
Sakai et al., Regul Pept 2002 : Here we have analyzed p38 MAPK activation by PACAP and the mechanism underlying this action of PACAP in PC12 cells ... PACAP increased phosphorylation of p38 MAPK with a bell shaped dose-response relationship and a maximal effect was obtained at 10 ( -8 ) M. PACAP ( 10 ( -8 ) M ) -induced p38 MAPK phosphorylation was already evident at 2.5 min, maximal at 5 min, and rapidly declined thereafter ... PACAP induced p38 MAPK phosphorylation was potently inhibited by depletion of Ca ( 2+ ) stores with thapsigargin and partially inhibited by the phospholipase C inhibitor U-73122, L-type voltage dependent calcium channel inhibitors nifedipine and nimodipine, and the Ca ( 2+ ) chelator EGTA, whereas the protein kinase C inhibitor calphostin C, the protein kinase A inhibitor H-89, the cAMP antagonist Rp-cAMP, and the nonselective cation channel blocker SKF96365 had no effect ... These results indicate that PACAP activates p38 MAPK in PC12 cells through activation of a phospholipase C, mobilization of intracellular Ca ( 2+ ) stores, and Ca ( 2+ ) influx through voltage dependent Ca ( 2+ ) channels, but not cyclic AMP dependent mechanisms
Fowkes et al., Mol Endocrinol 2003 : PACAP quickly activated MAPK ( within 5 min ) and also resulted in elevated levels of phospho-cAMP response element binding protein and phospho-SF-1, as judged by a specific antiphospho-S203 antibody ; this effect was blocked by the MAPK kinase inhibitor, UO126
Monaghan et al., J Neurochem 2008 (Neuroblastoma) : PACAP-38 and forskolin stimulated the activation of extracellular signal regulated kinase ( ERK ), mitogen activated protein kinase ( MAP ; p38 MAP kinase ) and c-Jun N-terminal kinase (JNK)
Moroo et al., Brain Res 1998 : In the present study, we examined the effect of PACAP on the activation of mitogen activated protein kinase ( MAPK ), one of the important intracellular signals for the proliferation, and compared it with that of epidermal growth factor (EGF) ... These data clearly demonstrated that PACAP stimulates MAPK in both a PKA- and a PKC independent manner in cultured rat astrocytes