Gene interactions and pathways from curated databases and text-mining
Int J Mol Med 2005, PMID: 15647850

High-dose hydrocortisone reduces expression of the pro-inflammatory chemokines CXCL8 and CXCL10 in SARS coronavirus-infected intestinal cells.

Cinatl, Jindrich; Michaelis, Martin; Morgenstern, Birgit; Doerr, Hans Wilhelm

Clinical observations and our high-density oligonucleotide microarray results demonstrated increased expression of proinflammatory chemokines after SARS-CoV infection. Here, we investigated the influence of SARS-CoV infection on CXCL8 (interleukin 8) and CXCL10 (interferon-gamma-inducible protein 10) in human intestinal epithelial (Caco2) cells. RT-PCR and ELISA showed time-dependent up-regulation of both chemokines after SARS-CoV infection. Electric mobility shift assay revealed increased DNA binding activity of the cellular transcription factors activator protein 1 (AP-1) and nuclear factor (B (NF-kappaB) in SARS-CoV infected cells. High hydrocortisone concentrations (> or =50 microg/ml) completely prevented increased DNA binding activity of AP-1 and NF-kappaB and inhibited up-regulation of CXCL8 and CXCL10, but did not reduce chemokine expression to basal levels. Ribavirin that does not inhibit SARS-CoV replication in Vero cells inhibited SARS-CoV replication in Caco2 cells at therapeutical concentrations. Hydrocortisone neither influenced SARS-CoV titres alone nor in combination with ribavirin. Our results show that corticosteroids may be of limited benefit in the suppression of chemokine production by SARS-CoV-infected cells.

Diseases/Pathways annotated by Medline MESH: Colonic Neoplasms, Inflammation, Severe Acute Respiratory Syndrome
Document information provided by NCBI PubMed

Text Mining Data

CXCL8 ⊣ AP-1: " High hydrocortisone concentrations ( > or =50 microg/ml ) completely prevented increased DNA binding activity of AP-1 and NF-kappaB and inhibited up-regulation of CXCL8 and CXCL10, but did not reduce chemokine expression to basal levels "

CXCL10 ⊣ AP-1: " High hydrocortisone concentrations ( > or =50 microg/ml ) completely prevented increased DNA binding activity of AP-1 and NF-kappaB and inhibited up-regulation of CXCL8 and CXCL10 , but did not reduce chemokine expression to basal levels "

CXCL10 ⊣ NF-kappaB: " High hydrocortisone concentrations ( > or =50 microg/ml ) completely prevented increased DNA binding activity of AP-1 and NF-kappaB and inhibited up-regulation of CXCL8 and CXCL10 , but did not reduce chemokine expression to basal levels "

CXCL8 ⊣ NF-kappaB: " High hydrocortisone concentrations ( > or =50 microg/ml ) completely prevented increased DNA binding activity of AP-1 and NF-kappaB and inhibited up-regulation of CXCL8 and CXCL10, but did not reduce chemokine expression to basal levels "

Manually curated Databases

No curated data.