Human Gene WTAP (ENST00000621533.5_5) from GENCODE V47lift37
  Description: WT1 associated protein, transcript variant 4 (from RefSeq NM_001270531.2)
Gencode Transcript: ENST00000621533.5_5
Gencode Gene: ENSG00000146457.16_9
Transcript (Including UTRs)
   Position: hg19 chr6:160,148,538-160,177,351 Size: 28,814 Total Exon Count: 8 Strand: +
Coding Region
   Position: hg19 chr6:160,157,298-160,176,643 Size: 19,346 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:160,148,538-160,177,351)mRNA (may differ from genome)Protein (396 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MalacardsMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: FL2D_HUMAN
DESCRIPTION: RecName: Full=Pre-mRNA-splicing regulator WTAP; AltName: Full=Female-lethal(2)D homolog; Short=hFL(2)D; AltName: Full=WT1-associated protein; AltName: Full=Wilms tumor 1-associating protein;
FUNCTION: Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation.
SUBUNIT: Interacts with WT1.
SUBCELLULAR LOCATION: Nucleus, nucleolus.
TISSUE SPECIFICITY: Ubiquitously expressed.
INDUCTION: In smooth muscle cells, up-regulated after serum withdrawal, when cells become mature and non proliferative.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the fl(2)d family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: WTAP
Diseases sorted by gene-association score: wilms tumor susceptibility-5 (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -106.40206-0.517 Picture PostScript Text
3' UTR -140.00708-0.198 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  Pfam Domains:
PF17098 - WTAP/Mum2p family

ModBase Predicted Comparative 3D Structure on Q15007
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0000381 regulation of alternative mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0007049 cell cycle
GO:0008380 RNA splicing
GO:0080009 mRNA methylation

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0016607 nuclear speck
GO:0031965 nuclear membrane
GO:0036396 MIS complex


-  Descriptions from all associated GenBank mRNAs
  AK127822 - Homo sapiens cDNA FLJ45925 fis, clone PLACE6016030, highly similar to Wilms' tumor 1-associating protein.
AK290854 - Homo sapiens cDNA FLJ76151 complete cds, highly similar to Homo sapiens Wilms tumor 1 associated protein (WTAP), transcript variant 1, mRNA.
LF385539 - JP 2014500723-A/193042: Polycomb-Associated Non-Coding RNAs.
AF374416 - Homo sapiens putative pre-mRNA splicing regulator female-lethal(2D) mRNA, complete cds.
BC069192 - Homo sapiens Wilms tumor 1 associated protein, mRNA (cDNA clone MGC:78409 IMAGE:4653831), complete cds.
D14661 - Homo sapiens KIAA0105 mRNA, complete cds.
CU686707 - Synthetic construct Homo sapiens gateway clone IMAGE:100023137 5' read WTAP mRNA.
HQ447554 - Synthetic construct Homo sapiens clone IMAGE:100070893; CCSB014864_01 Wilms tumor 1 associated protein (WTAP) gene, encodes complete protein.
AJ276706 - Homo sapiens partial mRNA for WTAP protein.
AL583911 - Homo sapiens mRNA; cDNA DKFZp761K0722 (from clone DKFZp761K0722).
MA621116 - JP 2018138019-A/193042: Polycomb-Associated Non-Coding RNAs.
BC000383 - Homo sapiens Wilms tumor 1 associated protein, mRNA (cDNA clone IMAGE:2820936), complete cds.
BC004432 - Homo sapiens Wilms tumor 1 associated protein, mRNA (cDNA clone IMAGE:2820936), complete cds.
BC028180 - Homo sapiens Wilms tumor 1 associated protein, mRNA (cDNA clone IMAGE:5399821), partial cds.
JD125871 - Sequence 106895 from Patent EP1572962.
KJ892811 - Synthetic construct Homo sapiens clone ccsbBroadEn_02205 WTAP gene, encodes complete protein.
LF340293 - JP 2014500723-A/147796: Polycomb-Associated Non-Coding RNAs.
LF340292 - JP 2014500723-A/147795: Polycomb-Associated Non-Coding RNAs.
LF340290 - JP 2014500723-A/147793: Polycomb-Associated Non-Coding RNAs.
AF277190 - Homo sapiens PNAS-132 mRNA, complete cds.
LF340287 - JP 2014500723-A/147790: Polycomb-Associated Non-Coding RNAs.
LF322405 - JP 2014500723-A/129908: Polycomb-Associated Non-Coding RNAs.
LF340286 - JP 2014500723-A/147789: Polycomb-Associated Non-Coding RNAs.
MA557982 - JP 2018138019-A/129908: Polycomb-Associated Non-Coding RNAs.
MA575870 - JP 2018138019-A/147796: Polycomb-Associated Non-Coding RNAs.
MA575869 - JP 2018138019-A/147795: Polycomb-Associated Non-Coding RNAs.
MA575867 - JP 2018138019-A/147793: Polycomb-Associated Non-Coding RNAs.
MA575864 - JP 2018138019-A/147790: Polycomb-Associated Non-Coding RNAs.
MA575863 - JP 2018138019-A/147789: Polycomb-Associated Non-Coding RNAs.
CR627456 - Homo sapiens mRNA; cDNA DKFZp686F20131 (from clone DKFZp686F20131).
AK131393 - Homo sapiens cDNA FLJ16480 fis, clone BRTHA2016318, highly similar to WTAP protein.
LF340279 - JP 2014500723-A/147782: Polycomb-Associated Non-Coding RNAs.
LF340278 - JP 2014500723-A/147781: Polycomb-Associated Non-Coding RNAs.
LF340277 - JP 2014500723-A/147780: Polycomb-Associated Non-Coding RNAs.
LF340276 - JP 2014500723-A/147779: Polycomb-Associated Non-Coding RNAs.
LF340275 - JP 2014500723-A/147778: Polycomb-Associated Non-Coding RNAs.
JD512117 - Sequence 493141 from Patent EP1572962.
JD221159 - Sequence 202183 from Patent EP1572962.
JD180664 - Sequence 161688 from Patent EP1572962.
JD241101 - Sequence 222125 from Patent EP1572962.
MA575856 - JP 2018138019-A/147782: Polycomb-Associated Non-Coding RNAs.
MA575855 - JP 2018138019-A/147781: Polycomb-Associated Non-Coding RNAs.
MA575854 - JP 2018138019-A/147780: Polycomb-Associated Non-Coding RNAs.
MA575853 - JP 2018138019-A/147779: Polycomb-Associated Non-Coding RNAs.
MA575852 - JP 2018138019-A/147778: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q15007 (Reactome details) participates in the following event(s):

R-HSA-72095 Internal Methylation of mRNA
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-8953854 Metabolism of RNA

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000621533.1, ENST00000621533.2, ENST00000621533.3, ENST00000621533.4, FL2D_HUMAN, KIAA0105 , NM_001270531, Q15007, Q5TCL8, Q5TCL9, Q96T28, Q9BYJ7, Q9H4E2, uc327trz.1, uc327trz.2, WTAP
UCSC ID: ENST00000621533.5_5
RefSeq Accession: NM_001270531.2
Protein: Q15007 (aka FL2D_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.