Human Gene TRIM69 (ENST00000329464.9_5) from GENCODE V47lift37
  Description: tripartite motif containing 69, transcript variant a (from RefSeq NM_182985.5)
Gencode Transcript: ENST00000329464.9_5
Gencode Gene: ENSG00000185880.14_10
Transcript (Including UTRs)
   Position: hg19 chr15:45,028,726-45,060,025 Size: 31,300 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr15:45,028,903-45,059,970 Size: 31,068 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:45,028,726-45,060,025)mRNA (may differ from genome)Protein (500 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TRI69_HUMAN
DESCRIPTION: RecName: Full=Tripartite motif-containing protein 69; AltName: Full=RFP-like domain-containing protein trimless; AltName: Full=RING finger protein 36;
FUNCTION: May play a role in apoptosis (By similarity).
SUBUNIT: Interacts with PML (By similarity).
SUBCELLULAR LOCATION: Nucleus speckle (By similarity).
PTM: Phosphorylated. Phosphorylation is necessary for nuclear localization (By similarity).
SIMILARITY: Belongs to the TRIM/RBCC family.
SIMILARITY: Contains 1 B30.2/SPRY domain.
SIMILARITY: Contains 1 RING-type zinc finger.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.76 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 192.84 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -39.70177-0.224 Picture PostScript Text
3' UTR -1.6055-0.029 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001870 - B30.2/SPRY
IPR003879 - Butyrophylin
IPR008985 - ConA-like_lec_gl_sf
IPR006574 - PRY
IPR018355 - SPla/RYanodine_receptor_subgr
IPR003877 - SPRY_rcpt
IPR018957 - Znf_C3HC4_RING-type
IPR001841 - Znf_RING
IPR013083 - Znf_RING/FYVE/PHD
IPR017907 - Znf_RING_CS

Pfam Domains:
PF00097 - Zinc finger, C3HC4 type (RING finger)
PF00622 - SPRY domain
PF13445 - RING-type zinc-finger
PF13765 - SPRY-associated domain
PF13923 - Zinc finger, C3HC4 type (RING finger)
PF15227 - zinc finger of C3HC4-type, RING

SCOP Domains:
48239 - Terpenoid cyclases/Protein prenyltransferases
49899 - Concanavalin A-like lectins/glucanases
57184 - Growth factor receptor domain
57845 - B-box zinc-binding domain
57850 - RING/U-box

ModBase Predicted Comparative 3D Structure on Q86WT6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0000209 protein polyubiquitination
GO:0006915 apoptotic process
GO:0016567 protein ubiquitination

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016607 nuclear speck


-  Descriptions from all associated GenBank mRNAs
  DQ232883 - Homo sapiens tripartite motif protein 69 (TRIM69) mRNA, complete cds.
AK292252 - Homo sapiens cDNA FLJ76057 complete cds, highly similar to Homo sapiens ring finger protein 36, transcript variant a, mRNA.
BC047945 - Homo sapiens tripartite motif-containing 69, mRNA (cDNA clone MGC:50818 IMAGE:5744224), complete cds.
AL360161 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1621663.
AF302088 - Homo sapiens TRIMLESS alpha (TRIMLESS) mRNA, complete cds.
AF302089 - Homo sapiens TRIMLESS beta (TRIMLESS) mRNA, complete cds.
JD131568 - Sequence 112592 from Patent EP1572962.
JD247448 - Sequence 228472 from Patent EP1572962.
JD152656 - Sequence 133680 from Patent EP1572962.
JD559463 - Sequence 540487 from Patent EP1572962.
BC024199 - Homo sapiens tripartite motif-containing 69, mRNA (cDNA clone IMAGE:4822946), partial cds.
JD464742 - Sequence 445766 from Patent EP1572962.
JD150701 - Sequence 131725 from Patent EP1572962.
JD254538 - Sequence 235562 from Patent EP1572962.
JD133697 - Sequence 114721 from Patent EP1572962.
JD154902 - Sequence 135926 from Patent EP1572962.
BC033314 - Homo sapiens tripartite motif-containing 69, mRNA (cDNA clone MGC:35082 IMAGE:5166812), complete cds.
JD111230 - Sequence 92254 from Patent EP1572962.
JD226707 - Sequence 207731 from Patent EP1572962.
JD174161 - Sequence 155185 from Patent EP1572962.
JD104217 - Sequence 85241 from Patent EP1572962.
JD400127 - Sequence 381151 from Patent EP1572962.
AY305385 - Homo sapiens HSD-34 mRNA, complete cds.
JD512091 - Sequence 493115 from Patent EP1572962.
AK226115 - Homo sapiens mRNA for ring finger protein 36 isoform a variant, clone: hh00788.
CU689624 - Synthetic construct Homo sapiens gateway clone IMAGE:100019011 5' read TRIM69 mRNA.
EU446573 - Synthetic construct Homo sapiens clone IMAGE:100070084; IMAGE:100011782; FLH257457.01L tripartite motif-containing 69 (TRIM69) gene, encodes complete protein.
EU832108 - Synthetic construct Homo sapiens clone HAIB:100067137; DKFZo008B1025 tripartite motif-containing 69 protein (TRIM69) gene, encodes complete protein.
EU832202 - Synthetic construct Homo sapiens clone HAIB:100067231; DKFZo004B1026 tripartite motif-containing 69 protein (TRIM69) gene, encodes complete protein.
KJ895549 - Synthetic construct Homo sapiens clone ccsbBroadEn_04943 TRIM69 gene, encodes complete protein.
JD419063 - Sequence 400087 from Patent EP1572962.
JD248124 - Sequence 229148 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q86WT6 (Reactome details) participates in the following event(s):

R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983147 Release of E3 from polyubiquitinated substrate
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: A8MX03, ENST00000329464.1, ENST00000329464.2, ENST00000329464.3, ENST00000329464.4, ENST00000329464.5, ENST00000329464.6, ENST00000329464.7, ENST00000329464.8, HSD-34, HSD34, NM_182985, Q309B0, Q4G1A5, Q6W897, Q86WT6, Q8IYY3, Q8WY16, Q8WY17, RNF36, TRI69_HUMAN, uc317ste.1, uc317ste.2
UCSC ID: ENST00000329464.9_5
RefSeq Accession: NM_182985.5
Protein: Q86WT6 (aka TRI69_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.