ID:CHP3_HUMAN DESCRIPTION: RecName: Full=Calcineurin B homologous protein 3; AltName: Full=Tescalcin; Short=TSC; FUNCTION: Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Promotes the maturation, transport, cell surface stability and exchange activity of SLC9A1/NHE1 at the plasma membrane. Promotes the induction of hematopoietic stem cell differentiation toward megakaryocytic lineage. Essential for the coupling of ERK cascade activation with the expression of ETS family genes in megakaryocytic differentiation. Also involved in granulocytic differentiation in a ERK-dependent manner. Inhibits the phosphatase activity of calcineurin. COFACTOR: Magnesium. Calcium-binding mediates the calcium-induced conformational change (By similarity). Calcium. SUBUNIT: Monomer. Homodimer; disulfide-linked (By similarity). Interacts with SLC9A1/NHE1 (via juxtamembrane region of the cytoplasmic C-terminus); the interaction enable an optimal Na(+)/H(+) exchange activity. INTERACTION: Q9NP66:HMG20A; NbExp=2; IntAct=EBI-740653, EBI-740641; SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Membrane (By similarity). Cell membrane. Cell projection, lamellipodium (By similarity). Cell projection, ruffle membrane (By similarity). Note=Colocalizes with SLC9A1 at the plasma membrane. TISSUE SPECIFICITY: Expressed in mature megakaryocytes and polymorphonuclear granulocytes (at protein level). Abundantly expressed in heart. Also expressed at a lower level in adult testis and salivary gland, and in the placenta. DEVELOPMENTAL STAGE: Strongly up-regulated in K562 cells treated by PMA to promote megakaryocytic differentiation, but not when treated by DMSO to promote granulocytic differentiation or by hemin to promote erythroid differentiation (at protein level). INDUCTION: Up-regulated during granulocytic differentiation in a ERK-dependent manner (is mediated by activation of ERK) (at protein level). Up-regulated during the differentiation and maturation of primary megakaryocytes. Down-regulated during monocytic-macrophage differentiation in a ERK-dependent manner. SIMILARITY: Belongs to the calcineurin regulatory subunit family. CHP subfamily. SIMILARITY: Contains 1 EF-hand domain. CAUTION: Although PubMed:12809501 reports that TESC results in a decrease in transporter activity of human SLC9A1, studies with rat SLC9A1 (PubMed:18321853) show that TESC-binding results in the maturation and accumulation of SLC9A1 at the cell surface. SEQUENCE CAUTION: Sequence=BAA91288.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=EAW98101.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q96BS2
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0006469 negative regulation of protein kinase activity GO:0008285 negative regulation of cell proliferation GO:0010628 positive regulation of gene expression GO:0015031 protein transport GO:0030154 cell differentiation GO:0030854 positive regulation of granulocyte differentiation GO:0032417 positive regulation of sodium:proton antiporter activity GO:0033628 regulation of cell adhesion mediated by integrin GO:0043086 negative regulation of catalytic activity GO:0045654 positive regulation of megakaryocyte differentiation GO:0045893 positive regulation of transcription, DNA-templated GO:0050821 protein stabilization GO:0051604 protein maturation GO:0071300 cellular response to retinoic acid GO:0072659 protein localization to plasma membrane
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
