Human Gene SIKE1 (ENST00000060969.6_9) from GENCODE V47lift37
  Description: suppressor of IKBKE 1, transcript variant 4 (from RefSeq NR_049742.2)
Gencode Transcript: ENST00000060969.6_9
Gencode Gene: ENSG00000052723.12_9
Transcript (Including UTRs)
   Position: hg19 chr1:115,312,100-115,323,306 Size: 11,207 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr1:115,316,892-115,323,228 Size: 6,337 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:115,312,100-115,323,306)mRNA (may differ from genome)Protein (207 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SIKE1_HUMAN
DESCRIPTION: RecName: Full=Suppressor of IKBKE 1; AltName: Full=Suppressor of IKK-epsilon;
FUNCTION: Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways.
SUBUNIT: Interacts with IKBKE and TBK1 via its coiled coil region. Interaction with TBK1 is disrupted upon viral infection or TLR3 stimulation.
INTERACTION: Q9Y228:TRAF3IP3; NbExp=2; IntAct=EBI-1773646, EBI-765817;
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and leukocytes. Present in all cell lines tested (at protein level).
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the SIKE family.
SEQUENCE CAUTION: Sequence=CAI18824.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.33 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 245.95 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -32.3078-0.414 Picture PostScript Text
3' UTR -1096.904792-0.229 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008555 - SIKE

Pfam Domains:
PF05769 - SIKE family

SCOP Domains:
46966 - Spectrin repeat
160459 - BLRF2-like

ModBase Predicted Comparative 3D Structure on Q9BRV8
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0017048 Rho GTPase binding
GO:0019901 protein kinase binding

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  CR749219 - Homo sapiens mRNA; cDNA DKFZp686A0768 (from clone DKFZp686A0768).
CR936771 - Homo sapiens mRNA; cDNA DKFZp686A0668 (from clone DKFZp686A0668).
LF206268 - JP 2014500723-A/13771: Polycomb-Associated Non-Coding RNAs.
AK126316 - Homo sapiens cDNA FLJ44339 fis, clone TRACH3004840.
LF352819 - JP 2014500723-A/160322: Polycomb-Associated Non-Coding RNAs.
AK024821 - Homo sapiens cDNA: FLJ21168 fis, clone CAS10874.
JD183005 - Sequence 164029 from Patent EP1572962.
JD240501 - Sequence 221525 from Patent EP1572962.
JD363050 - Sequence 344074 from Patent EP1572962.
LF352818 - JP 2014500723-A/160321: Polycomb-Associated Non-Coding RNAs.
JD545661 - Sequence 526685 from Patent EP1572962.
LF352817 - JP 2014500723-A/160320: Polycomb-Associated Non-Coding RNAs.
BC005934 - Homo sapiens suppressor of IKK epsilon, mRNA (cDNA clone MGC:14549 IMAGE:4249034), complete cds.
LF352815 - JP 2014500723-A/160318: Polycomb-Associated Non-Coding RNAs.
JD281106 - Sequence 262130 from Patent EP1572962.
JD227319 - Sequence 208343 from Patent EP1572962.
LF352813 - JP 2014500723-A/160316: Polycomb-Associated Non-Coding RNAs.
JD252442 - Sequence 233466 from Patent EP1572962.
JD285649 - Sequence 266673 from Patent EP1572962.
JD233496 - Sequence 214520 from Patent EP1572962.
JD480634 - Sequence 461658 from Patent EP1572962.
LF352812 - JP 2014500723-A/160315: Polycomb-Associated Non-Coding RNAs.
AK225734 - Homo sapiens mRNA for hypothetical protein LOC80143 variant, clone: TST04964.
JD074012 - Sequence 55036 from Patent EP1572962.
JD419075 - Sequence 400099 from Patent EP1572962.
JD308404 - Sequence 289428 from Patent EP1572962.
EU832701 - Synthetic construct Homo sapiens clone HAIB:100067730; DKFZo004C0632 suppressor of IKK epsilon protein (SIKE) gene, encodes complete protein.
AB590611 - Synthetic construct DNA, clone: pFN21AE1707, Homo sapiens SIKE1 gene for suppressor of IKBKE 1, without stop codon, in Flexi system.
LF352811 - JP 2014500723-A/160314: Polycomb-Associated Non-Coding RNAs.
JD218980 - Sequence 200004 from Patent EP1572962.
MA588396 - JP 2018138019-A/160322: Polycomb-Associated Non-Coding RNAs.
MA588395 - JP 2018138019-A/160321: Polycomb-Associated Non-Coding RNAs.
MA588394 - JP 2018138019-A/160320: Polycomb-Associated Non-Coding RNAs.
MA588392 - JP 2018138019-A/160318: Polycomb-Associated Non-Coding RNAs.
MA588390 - JP 2018138019-A/160316: Polycomb-Associated Non-Coding RNAs.
MA588389 - JP 2018138019-A/160315: Polycomb-Associated Non-Coding RNAs.
MA588388 - JP 2018138019-A/160314: Polycomb-Associated Non-Coding RNAs.
MA441845 - JP 2018138019-A/13771: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BRV8 (Reactome details) participates in the following event(s):

R-HSA-918225 TBK1/IKK epsilon complex interacts with MAVS bound TRAF3
R-HSA-933527 Recruitment of TBK1/IKK epsilon complex to TANK:TRAF6
R-HSA-918233 TRAF3-dependent IRF activation pathway
R-HSA-933541 TRAF6 mediated IRF7 activation
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-168249 Innate Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000060969.1, ENST00000060969.2, ENST00000060969.3, ENST00000060969.4, ENST00000060969.5, NR_049742, Q5TEZ7, Q5TEZ9, Q68DZ4, Q9BRV8, Q9H778, SIKE, SIKE1_HUMAN, uc317cai.1, uc317cai.2
UCSC ID: ENST00000060969.6_9
RefSeq Accession: NM_025073.3
Protein: Q9BRV8 (aka SIKE1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.