ID:PSD10_HUMAN DESCRIPTION: RecName: Full=26S proteasome non-ATPase regulatory subunit 10; AltName: Full=26S proteasome regulatory subunit p28; AltName: Full=Gankyrin; AltName: Full=p28(GANK); FUNCTION: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module. FUNCTION: Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export. SUBUNIT: Part of transient complex containing PSMD10, PSMC4, PSMC5 and PAAF1 formed during the assembly of the 26S proteasome. Stays associated throughout the assembly of the PA700/19S RC and is released upon association with the 20S core. Interacts with PSMC4. Interacts with RB1. Interacts with CDK4. Interacts with MDM2. Interacts with RELA. Associates with a CDK4:CCND2 serine/threonine kinase complex. INTERACTION: P43358:MAGEA4; NbExp=5; IntAct=EBI-752185, EBI-743122; P43686:PSMC4; NbExp=8; IntAct=EBI-752185, EBI-743997; SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Overexpressed in hepatocellular carcinomas. SIMILARITY: Contains 7 ANK repeats. CAUTION: Was initially identified as a genuine component of the 26S proteasome.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O75832
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0005515 protein binding GO:0008134 transcription factor binding
Biological Process: GO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0006915 apoptotic process GO:0007253 cytoplasmic sequestering of NF-kappaB GO:0016579 protein deubiquitination GO:0030307 positive regulation of cell growth GO:0031398 positive regulation of protein ubiquitination GO:0032088 negative regulation of NF-kappaB transcription factor activity GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0043066 negative regulation of apoptotic process GO:0043409 negative regulation of MAPK cascade GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator GO:0043687 post-translational protein modification GO:0045737 positive regulation of cyclin-dependent protein serine/threonine kinase activity GO:0070682 proteasome regulatory particle assembly GO:0090201 negative regulation of release of cytochrome c from mitochondria
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
