Human Gene PIP4K2B (ENST00000619039.5_7) from GENCODE V47lift37
  Description: phosphatidylinositol-5-phosphate 4-kinase type 2 beta (from RefSeq NM_003559.5)
Gencode Transcript: ENST00000619039.5_7
Gencode Gene: ENSG00000276293.5_12
Transcript (Including UTRs)
   Position: hg19 chr17:36,921,944-36,955,809 Size: 33,866 Total Exon Count: 10 Strand: -
Coding Region
   Position: hg19 chr17:36,925,944-36,955,677 Size: 29,734 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:36,921,944-36,955,809)mRNA (may differ from genome)Protein (416 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PI42B_HUMAN
DESCRIPTION: RecName: Full=Phosphatidylinositol 5-phosphate 4-kinase type-2 beta; EC=2.7.1.149; AltName: Full=1-phosphatidylinositol 5-phosphate 4-kinase 2-beta; AltName: Full=Diphosphoinositide kinase 2-beta; AltName: Full=Phosphatidylinositol 5-phosphate 4-kinase type II beta; Short=PI(5)P 4-kinase type II beta; Short=PIP4KII-beta; AltName: Full=PtdIns(5)P-4-kinase isoform 2-beta;
FUNCTION: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.
CATALYTIC ACTIVITY: ATP + 1-phosphatidyl-1D-myo-inositol 5- phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=30 uM for phosphatidylinositol-5- phosphate; Vmax=0.2 pmol/min/ug enzyme;
SUBUNIT: Homodimer. Binds TNFRSF1A. Interacts with PIP4K2A. Interaction with PIP4K2A suppresses ubiquitination by the SPOP/ CUL3 complex.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein (By similarity). Cell membrane; Peripheral membrane protein (By similarity). Nucleus. Cytoplasm. Note=Associated with the plasma membrane and the endoplasmic reticulum (By similarity).
TISSUE SPECIFICITY: Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver.
PTM: Ubiquitinated by the SPOP/CUL3 complex. Ubiquitination is stimulated by PtdIns5P levels.
SIMILARITY: Contains 1 PIPK domain.

-  Primer design for this transcript
 

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-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -76.90132-0.583 Picture PostScript Text
3' UTR -1404.404000-0.351 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR023610 - PInositol-4-P-5-kinase
IPR002498 - PInositol-4-P-5-kinase_core
IPR016034 - PInositol-4P-5-kinase_core_sub

Pfam Domains:
PF01504 - Phosphatidylinositol-4-phosphate 5-Kinase

SCOP Domains:
56104 - SAICAR synthase-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1BO1 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P78356
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGD  WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0005057 signal transducer activity, downstream of receptor
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016307 phosphatidylinositol phosphate kinase activity
GO:0016308 1-phosphatidylinositol-4-phosphate 5-kinase activity
GO:0016309 1-phosphatidylinositol-5-phosphate 4-kinase activity
GO:0016740 transferase activity
GO:0042803 protein homodimerization activity
GO:0052811 1-phosphatidylinositol-3-phosphate 4-kinase activity

Biological Process:
GO:0006644 phospholipid metabolic process
GO:0006661 phosphatidylinositol biosynthetic process
GO:0007166 cell surface receptor signaling pathway
GO:0010506 regulation of autophagy
GO:0014066 regulation of phosphatidylinositol 3-kinase signaling
GO:0016310 phosphorylation
GO:0046488 phosphatidylinositol metabolic process
GO:0046854 phosphatidylinositol phosphorylation
GO:2000786 positive regulation of autophagosome assembly

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005776 autophagosome
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  BC073896 - Homo sapiens cDNA clone IMAGE:4158112, partial cds.
BC094858 - Homo sapiens cDNA clone IMAGE:3535440.
DQ572189 - Homo sapiens piRNA piR-40301, complete sequence.
JD112951 - Sequence 93975 from Patent EP1572962.
JD156696 - Sequence 137720 from Patent EP1572962.
JD260794 - Sequence 241818 from Patent EP1572962.
U85245 - Human phosphatidylinositol-4-phosphate 5-kinase type II beta mRNA, complete cds.
JD041705 - Sequence 22729 from Patent EP1572962.
JD486606 - Sequence 467630 from Patent EP1572962.
JD210205 - Sequence 191229 from Patent EP1572962.
JD303779 - Sequence 284803 from Patent EP1572962.
JD074733 - Sequence 55757 from Patent EP1572962.
JD436398 - Sequence 417422 from Patent EP1572962.
JD540055 - Sequence 521079 from Patent EP1572962.
JD063326 - Sequence 44350 from Patent EP1572962.
JD512989 - Sequence 494013 from Patent EP1572962.
AK307169 - Homo sapiens cDNA, FLJ97117.
JD073573 - Sequence 54597 from Patent EP1572962.
JD400980 - Sequence 382004 from Patent EP1572962.
JD389411 - Sequence 370435 from Patent EP1572962.
JD555760 - Sequence 536784 from Patent EP1572962.
JD484170 - Sequence 465194 from Patent EP1572962.
JD318284 - Sequence 299308 from Patent EP1572962.
JD102533 - Sequence 83557 from Patent EP1572962.
JD555757 - Sequence 536781 from Patent EP1572962.
JD480833 - Sequence 461857 from Patent EP1572962.
JD124170 - Sequence 105194 from Patent EP1572962.
JD540426 - Sequence 521450 from Patent EP1572962.
JD339679 - Sequence 320703 from Patent EP1572962.
JD176021 - Sequence 157045 from Patent EP1572962.
JD417349 - Sequence 398373 from Patent EP1572962.
JD551801 - Sequence 532825 from Patent EP1572962.
JD415591 - Sequence 396615 from Patent EP1572962.
JD399188 - Sequence 380212 from Patent EP1572962.
JD475906 - Sequence 456930 from Patent EP1572962.
JD383971 - Sequence 364995 from Patent EP1572962.
JD335692 - Sequence 316716 from Patent EP1572962.
JD288377 - Sequence 269401 from Patent EP1572962.
JD555494 - Sequence 536518 from Patent EP1572962.
JD095794 - Sequence 76818 from Patent EP1572962.
JD516507 - Sequence 497531 from Patent EP1572962.
JD093016 - Sequence 74040 from Patent EP1572962.
JD180409 - Sequence 161433 from Patent EP1572962.
JD399234 - Sequence 380258 from Patent EP1572962.
JD055530 - Sequence 36554 from Patent EP1572962.
JD104963 - Sequence 85987 from Patent EP1572962.
JD513849 - Sequence 494873 from Patent EP1572962.
JD045708 - Sequence 26732 from Patent EP1572962.
JD090483 - Sequence 71507 from Patent EP1572962.
JD207447 - Sequence 188471 from Patent EP1572962.
JD327462 - Sequence 308486 from Patent EP1572962.
JD077016 - Sequence 58040 from Patent EP1572962.
JD162915 - Sequence 143939 from Patent EP1572962.
JD411705 - Sequence 392729 from Patent EP1572962.
BT019614 - Homo sapiens phosphatidylinositol-4-phosphate 5-kinase, type II, beta mRNA, complete cds.
AB529089 - Synthetic construct DNA, clone: pF1KB4223, Homo sapiens PIP5K2B gene for phosphatidylinositol-5-phosphate 4-kinase, type II, beta, without stop codon, in Flexi system.
BC027459 - Homo sapiens phosphatidylinositol-5-phosphate 4-kinase, type II, beta, mRNA (cDNA clone MGC:34571 IMAGE:3877995), complete cds.
CU693266 - Synthetic construct Homo sapiens gateway clone IMAGE:100016636 5' read PIP4K2B mRNA.
KJ901876 - Synthetic construct Homo sapiens clone ccsbBroadEn_11270 PIP4K2B gene, encodes complete protein.
JD133542 - Sequence 114566 from Patent EP1572962.
JD395988 - Sequence 377012 from Patent EP1572962.
JD542060 - Sequence 523084 from Patent EP1572962.
JD473134 - Sequence 454158 from Patent EP1572962.
JD408880 - Sequence 389904 from Patent EP1572962.
JD395968 - Sequence 376992 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-6351 - D-myo-inositol (1,4,5)-trisphosphate biosynthesis
PWY-6352 - 3-phosphoinositide biosynthesis
PWY-6371 - superpathway of inositol phosphate compounds

Reactome (by CSHL, EBI, and GO)

Protein P78356 (Reactome details) participates in the following event(s):

R-HSA-8877691 MAP2K6 phosphorylates PIP4K2B
R-HSA-8877692 PIN1 binds phosphorylated PIP4K2B
R-HSA-1676145 PI3P is phosphorylated to PI(3,4)P2 by PIP4K2/5K1 at the plasma membrane
R-HSA-1675776 PI5P is phosphorylated to PI(4,5)P2 by PIP4K2 dimers at the plasma membrane
R-HSA-6811522 PI5P is phosphorylated to PI(4,5)P2 by PIP4K2 dimers in the nucleus
R-HSA-6811555 PI5P Regulates TP53 Acetylation
R-HSA-1660499 Synthesis of PIPs at the plasma membrane
R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-8847453 Synthesis of PIPs in the nucleus
R-HSA-6804758 Regulation of TP53 Activity through Acetylation
R-HSA-1483255 PI Metabolism
R-HSA-199418 Negative regulation of the PI3K/AKT network
R-HSA-5633007 Regulation of TP53 Activity
R-HSA-1483257 Phospholipid metabolism
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-3700989 Transcriptional Regulation by TP53
R-HSA-556833 Metabolism of lipids
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-212436 Generic Transcription Pathway
R-HSA-1430728 Metabolism
R-HSA-162582 Signal Transduction
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000619039.1, ENST00000619039.2, ENST00000619039.3, ENST00000619039.4, NM_003559, P78356, PI42B_HUMAN, PIP4K2B , PIP5K2B, Q5U0E8, Q8TBP2, uc327sch.1, uc327sch.2
UCSC ID: ENST00000619039.5_7
RefSeq Accession: NM_003559.5
Protein: P78356 (aka PI42B_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
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