Human Gene PARP4 (ENST00000381989.4_7) from GENCODE V47lift37
  Description: poly(ADP-ribose) polymerase family member 4 (from RefSeq NM_006437.4)
Gencode Transcript: ENST00000381989.4_7
Gencode Gene: ENSG00000102699.6_10
Transcript (Including UTRs)
   Position: hg19 chr13:24,995,069-25,086,916 Size: 91,848 Total Exon Count: 34 Strand: -
Coding Region
   Position: hg19 chr13:24,995,257-25,077,914 Size: 82,658 Coding Exon Count: 33 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr13:24,995,069-25,086,916)mRNA (may differ from genome)Protein (1724 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PARP4_HUMAN
DESCRIPTION: RecName: Full=Poly [ADP-ribose] polymerase 4; Short=PARP-4; EC=2.4.2.30; AltName: Full=193 kDa vault protein; AltName: Full=ADP-ribosyltransferase diphtheria toxin-like 4; Short=ARTD4; AltName: Full=PARP-related/IalphaI-related H5/proline-rich; Short=PH5P; AltName: Full=Vault poly(ADP-ribose) polymerase; Short=VPARP;
CATALYTIC ACTIVITY: NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
SUBUNIT: Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1.
INTERACTION: Q9Q2G4:ORF (xeno); NbExp=3; IntAct=EBI-2623021, EBI-6248094;
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Also found in the nucleus, associated with mitotic spindles.
TISSUE SPECIFICITY: Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
SIMILARITY: Contains 1 BRCT domain.
SIMILARITY: Contains 1 PARP alpha-helical domain.
SIMILARITY: Contains 1 PARP catalytic domain.
SIMILARITY: Contains 1 VIT domain.
SIMILARITY: Contains 1 VWFA domain.
SEQUENCE CAUTION: Sequence=BAA11494.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 25.50 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 513.64 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -21.4074-0.289 Picture PostScript Text
3' UTR -22.40188-0.119 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001357 - BRCT_dom
IPR012317 - Poly(ADP-ribose)pol_cat_dom
IPR004102 - Poly(ADP-ribose)pol_reg_dom
IPR013694 - VIT
IPR002035 - VWF_A

Pfam Domains:
PF00092 - von Willebrand factor type A domain
PF00533 - BRCA1 C Terminus (BRCT) domain
PF00644 - Poly(ADP-ribose) polymerase catalytic domain
PF08487 - Vault protein inter-alpha-trypsin domain
PF13519 - von Willebrand factor type A domain
PF13757 - Vault protein inter-alpha-trypsin domain
PF13768 - von Willebrand factor type A domain
PF16589 - BRCT domain, a BRCA1 C-terminus domain

SCOP Domains:
47587 - Domain of poly(ADP-ribose) polymerase
52113 - BRCT domain
53300 - vWA-like
56399 - ADP-ribosylation

ModBase Predicted Comparative 3D Structure on Q9UKK3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003677 DNA binding
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0016757 transferase activity, transferring glycosyl groups
GO:0019899 enzyme binding

Biological Process:
GO:0006281 DNA repair
GO:0006464 cellular protein modification process
GO:0006471 protein ADP-ribosylation
GO:0006954 inflammatory response
GO:0006974 cellular response to DNA damage stimulus
GO:0008219 cell death
GO:0042493 response to drug
GO:0051972 regulation of telomerase activity

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005819 spindle
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005876 spindle microtubule
GO:0016020 membrane
GO:0070062 extracellular exosome
GO:1990904 ribonucleoprotein complex


-  Descriptions from all associated GenBank mRNAs
  AF158255 - Homo sapiens vault protein mRNA, complete cds.
D79999 - Homo sapiens KIAA0177 mRNA for KIAA0177 protein.
AF057160 - Homo sapiens putative poly(ADP-ribosyl) transferase (PARPL) mRNA, complete cds.
BC167791 - Synthetic construct Homo sapiens clone IMAGE:100068181, MGC:195798 poly (ADP-ribose) polymerase family, member 4 (PARP4) mRNA, encodes complete protein.
AB384443 - Synthetic construct DNA, clone: pF1KA0177, Homo sapiens PARP4 gene for poly [ADP-ribose] polymerase 4, complete cds, without stop codon, in Flexi system.
AK304399 - Homo sapiens cDNA FLJ61556 complete cds, highly similar to Poly (ADP-ribose) polymerase 4 (EC 2.4.2.30).
JD548637 - Sequence 529661 from Patent EP1572962.
JD021735 - Sequence 2759 from Patent EP1572962.
JD029946 - Sequence 10970 from Patent EP1572962.
BC017815 - Homo sapiens poly (ADP-ribose) polymerase family, member 4, mRNA (cDNA clone IMAGE:4618740), partial cds.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9UKK3 (Reactome details) participates in the following event(s):

R-HSA-197264 Nicotinamide salvaging
R-HSA-196807 Nicotinate metabolism
R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
R-HSA-196854 Metabolism of vitamins and cofactors
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ADPRTL1 , ENST00000381989.1, ENST00000381989.2, ENST00000381989.3, KIAA0177 , NM_006437, O75903, PARP4 , PARP4_HUMAN, PARPL, Q14682, Q5QNZ9, Q9H1M6, Q9UKK3, uc318qoz.1, uc318qoz.2
UCSC ID: ENST00000381989.4_7
RefSeq Accession: NM_006437.4
Protein: Q9UKK3 (aka PARP4_HUMAN or PPO4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.