Human Gene PARP15 (ENST00000464300.7_4) from GENCODE V47lift37
  Description: poly(ADP-ribose) polymerase family member 15, transcript variant 1 (from RefSeq NM_001113523.3)
Gencode Transcript: ENST00000464300.7_4
Gencode Gene: ENSG00000173200.13_7
Transcript (Including UTRs)
   Position: hg19 chr3:122,296,497-122,357,894 Size: 61,398 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg19 chr3:122,296,515-122,354,947 Size: 58,433 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Model InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr3:122,296,497-122,357,894)mRNA (may differ from genome)Protein (678 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PAR15_HUMAN
DESCRIPTION: RecName: Full=Poly [ADP-ribose] polymerase 15; Short=PARP-15; EC=2.4.2.30; AltName: Full=ADP-ribosyltransferase diphtheria toxin-like 7; Short=ARTD7; AltName: Full=B-aggressive lymphoma protein 3;
FUNCTION: Transcriptional repressor. Has ADP-ribosyltransferase activity.
CATALYTIC ACTIVITY: NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: Contains 2 Macro domains.
SIMILARITY: Contains 1 PARP catalytic domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 26.36 RPKM in Spleen
Total median expression: 81.93 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -2.6018-0.144 Picture PostScript Text
3' UTR -734.602947-0.249 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002589 - A1pp
IPR012317 - Poly(ADP-ribose)pol_cat_dom

Pfam Domains:
PF00644 - Poly(ADP-ribose) polymerase catalytic domain
PF01661 - Macro domain

SCOP Domains:
52949 - Macro domain-like
56399 - ADP-ribosylation

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3BLJ - X-ray MuPIT 3GEY - X-ray MuPIT 3KH6 - X-ray 3V2B - X-ray MuPIT 4F0E - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q460N3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001191 transcriptional repressor activity, RNA polymerase II transcription factor binding
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0005515 protein binding
GO:0016740 transferase activity
GO:0016757 transferase activity, transferring glycosyl groups
GO:0070403 NAD+ binding

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0070212 protein poly-ADP-ribosylation

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AK309863 - Homo sapiens cDNA, FLJ99904.
BX647656 - Homo sapiens mRNA; cDNA DKFZp781B0227 (from clone DKFZp781B0227).
DQ063586 - Homo sapiens B-aggressive lymphoma 3 (BAL3) mRNA, complete cds.
AK097515 - Homo sapiens cDNA FLJ40196 fis, clone TESTI2019794, highly similar to Homo sapiens poly (ADP-ribose) polymerase family, member 15 (PARP15), mRNA.
BC101701 - Homo sapiens poly (ADP-ribose) polymerase family, member 15, mRNA (cDNA clone MGC:126750 IMAGE:8069207), complete cds.
BC101703 - Homo sapiens poly (ADP-ribose) polymerase family, member 15, mRNA (cDNA clone MGC:126752 IMAGE:8069209), complete cds.
BC143573 - Homo sapiens poly (ADP-ribose) polymerase family, member 15, mRNA (cDNA clone MGC:177101 IMAGE:9052084), complete cds.
AK097916 - Homo sapiens cDNA FLJ40597 fis, clone THYMU2011118.
KJ895741 - Synthetic construct Homo sapiens clone ccsbBroadEn_05135 PARP15 gene, encodes complete protein.
JD049320 - Sequence 30344 from Patent EP1572962.
JD087309 - Sequence 68333 from Patent EP1572962.
JD432850 - Sequence 413874 from Patent EP1572962.
JD168420 - Sequence 149444 from Patent EP1572962.
JD041092 - Sequence 22116 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: BAL3 , ENST00000464300.1, ENST00000464300.2, ENST00000464300.3, ENST00000464300.4, ENST00000464300.5, ENST00000464300.6, J3KR47, NM_001113523, PAR15_HUMAN, PARP15 , Q460N3, Q8N1K3, uc321geg.1, uc321geg.2
UCSC ID: ENST00000464300.7_4
RefSeq Accession: NM_001113523.3
Protein: Q460N3 (aka PAR15_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.