ID:NCBP1_HUMAN DESCRIPTION: RecName: Full=Nuclear cap-binding protein subunit 1; AltName: Full=80 kDa nuclear cap-binding protein; Short=CBP80; Short=NCBP 80 kDa subunit; FUNCTION: Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. SUBUNIT: Component of the nuclear cap-binding complex (CBC), a heterodimer composed of NCBP1/CBP80 and NCBP2/CBP20 that interacts with m7GpppG-capped RNA. Found in a U snRNA export complex with RNUXA/PHAX, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA. Interaction with RNUXA/PHAX (By similarity). Heterodimer with NCBP2. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, ALYREF/THOC4 and/or REFBP2. Interacts with SRRT/ARS2, EIF4G2, IGF2BP1, HNRNPF, HNRNPH1, KIAA0427/CTIF, PARN, DROSHA, UPF1 and ALYREF/THOC4. May interact with EIF4G1; the interaction is however controversial since it is reported by PubMed:11340157, PubMed:15059963 and PubMed:15361857, but is not observed by PubMed:19648179. INTERACTION: P52298:NCBP2; NbExp=5; IntAct=EBI-464743, EBI-464729; Q9BY77:POLDIP3; NbExp=3; IntAct=EBI-464743, EBI-1776152; P62753:RPS6; NbExp=3; IntAct=EBI-464743, EBI-356625; SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. SIMILARITY: Belongs to the NCBP1 family. SIMILARITY: Contains 1 MIF4G domain. SEQUENCE CAUTION: Sequence=BAD92470.1; Type=Erroneous initiation; Sequence=CAI12863.1; Type=Erroneous gene model prediction;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q09161
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
LF206450 - JP 2014500723-A/13953: Polycomb-Associated Non-Coding RNAs. AK312807 - Homo sapiens cDNA, FLJ93236, highly similar to Homo sapiens nuclear cap binding protein subunit 1, 80kDa (NCBP1),mRNA. D32002 - Homo sapiens mRNA for nuclear cap binding protein, complete cds. BC001450 - Homo sapiens nuclear cap binding protein subunit 1, 80kDa, mRNA (cDNA clone MGC:2087 IMAGE:3138757), complete cds. AB209233 - Homo sapiens mRNA for nuclear cap binding protein subunit 1, 80kDa variant protein. X80030 - H.sapiens CBP80 mRNA. KJ905234 - Synthetic construct Homo sapiens clone ccsbBroadEn_14708 NCBP1 gene, encodes complete protein. AB384886 - Synthetic construct DNA, clone: pF1KB4031, Homo sapiens NCBP1 gene for nuclear cap-binding protein subunit 1, complete cds, without stop codon, in Flexi system. DQ895382 - Synthetic construct Homo sapiens clone IMAGE:100009842; FLH183828.01L; RZPDo839H01141D nuclear cap binding protein subunit 1, 80kDa (NCBP1) gene, encodes complete protein. EU176351 - Synthetic construct Homo sapiens clone IMAGE:100006436; FLH183834.01X; RZPDo839C10251D nuclear cap binding protein subunit 1, 80kDa (NCBP1) gene, encodes complete protein. MA442027 - JP 2018138019-A/13953: Polycomb-Associated Non-Coding RNAs. LF354544 - JP 2014500723-A/162047: Polycomb-Associated Non-Coding RNAs. MA590121 - JP 2018138019-A/162047: Polycomb-Associated Non-Coding RNAs. LF354554 - JP 2014500723-A/162057: Polycomb-Associated Non-Coding RNAs. LF354555 - JP 2014500723-A/162058: Polycomb-Associated Non-Coding RNAs. LF354558 - JP 2014500723-A/162061: Polycomb-Associated Non-Coding RNAs. LF354561 - JP 2014500723-A/162064: Polycomb-Associated Non-Coding RNAs. LF354562 - JP 2014500723-A/162065: Polycomb-Associated Non-Coding RNAs. JD564803 - Sequence 545827 from Patent EP1572962. JD202662 - Sequence 183686 from Patent EP1572962. DQ581037 - Homo sapiens piRNA piR-49149, complete sequence. JD434260 - Sequence 415284 from Patent EP1572962. JD430527 - Sequence 411551 from Patent EP1572962. JD529799 - Sequence 510823 from Patent EP1572962. JD435043 - Sequence 416067 from Patent EP1572962. JD307202 - Sequence 288226 from Patent EP1572962. JD323084 - Sequence 304108 from Patent EP1572962. JD401834 - Sequence 382858 from Patent EP1572962. JD401835 - Sequence 382859 from Patent EP1572962. MA590131 - JP 2018138019-A/162057: Polycomb-Associated Non-Coding RNAs. MA590132 - JP 2018138019-A/162058: Polycomb-Associated Non-Coding RNAs. MA590135 - JP 2018138019-A/162061: Polycomb-Associated Non-Coding RNAs. MA590138 - JP 2018138019-A/162064: Polycomb-Associated Non-Coding RNAs. MA590139 - JP 2018138019-A/162065: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q09161 (Reactome details) participates in the following event(s):
R-HSA-77094 Formation of cap binding complex (CBC) R-HSA-77587 Docking of Mature Replication Dependent Histone mRNA with the NPC R-HSA-77095 Recognition and binding of the mRNA cap by the cap-binding complex R-HSA-167089 Recognition and binding of the HIV-1 mRNA cap by the cap-binding complex R-HSA-6814885 CBCAP complex binds 7-methylguanosine cap of snRNA R-HSA-77594 Docking of the Mature intronless derived transcript derived mRNA, TAP and Aly/Ref at the NPC R-HSA-111439 Docking of Mature Histone mRNA complex:TAP at the NPC R-HSA-112381 Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex R-HSA-167084 Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex R-HSA-167191 Hyperphosphorylation (Ser2) of RNA Pol II CTD by the P-TEFb(Cyclin T1:Cdk9) complex R-HSA-77586 Cleavage of the 3'-end of Replication Dependent Histone Pre-mRNA R-HSA-77589 Recognition of AAUAAA sequence by CPSF R-HSA-77592 Cleavage of Intronless Pre-mRNA at 3'-end R-HSA-191825 Nuclear export of snRNA transcripts R-HSA-72103 Formation of pre-mRNPs R-HSA-75080 Formation of AT-AC A complex R-HSA-72095 Internal Methylation of mRNA R-HSA-8849157 TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally R-HSA-72185 mRNA polyadenylation R-HSA-72180 Cleavage of mRNA at the 3'-end R-HSA-112383 Hypophosphorylation of RNA Pol II CTD by FCP1P protein R-HSA-77584 Binding of SLBP to Replication-Dependent Histone Pre-mRNA R-HSA-111438 Recruitment of U7 snRNP:ZFP100 complex to the Histone Pre-mRNA R-HSA-77593 Cleavage and polyadenylation of Intronless Pre-mRNA R-HSA-113407 DSIF complex binds to RNA Pol II (hypophosphorylated) R-HSA-75096 Docking of the TAP:EJC Complex with the NPC R-HSA-159101 NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B) R-HSA-111437 Cleavage of the 3'-end of the Histone Pre-mRNA R-HSA-167072 Hypophosphorylation of RNA Pol II CTD by FCP1P protein R-HSA-167083 DSIF complex binds to RNA Pol II (hypophosphorylated) R-HSA-6803523 PTB and hnRNPA1 bind FGFR2 pre-mRNA to repress IIIb splicing and promote formation of FGFR2c mRNA R-HSA-6803527 ESRP1 and 2 bind FGFR2 pre-mRNA to promote FGFR2b maturation and expression R-HSA-77590 Recruitment of CstF to the CPSF Bound Pre-mRNA R-HSA-75098 mRNP complex dissociates from cytosolic face of NPC R-HSA-72107 Formation of the Spliceosomal E complex R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-75081 Formation of AT-AC B Complex R-HSA-75082 ATAC spliceosome mediated Lariat formation,5' splice site cleavage R-HSA-75079 Formation of AT-AC C complex R-HSA-75083 ATAC spliceosome mediated 3' splice site cleavage, exon ligation R-HSA-156661 Formation of Exon Junction Complex R-HSA-77585 Recruitment of U7 snRNP:ZFP100 complex to the SLBP Bound Pre-mRNA R-HSA-113402 Formation of DSIF:NELF:early elongation complex R-HSA-167085 Formation of DSIF:NELF:HIV-1 early elongation complex R-HSA-77591 Binding of Cleavage factors and Poly(A)Polymerase to the CstF:CPSF:Pre-mRNA Complex R-HSA-927832 UPF1 binds an mRNP with a termination codon preceding an Exon Junction Complex R-HSA-927789 Formation of UPF1:eRF3 complex on mRNA with a premature termination codon and no Exon Junction Complex R-HSA-113409 Abortive termination of early transcription elongation by DSIF:NELF R-HSA-167087 Limited elongation of the HIV-1 transcript R-HSA-167478 Abortive termination of HIV-1 early transcription elongation by DSIF:NELF R-HSA-167147 Separation of abortive HIV-1 transcript from template R-HSA-927889 SMG1 phosphorylates UPF1 (enhanced by Exon Junction Complex) R-HSA-72086 mRNA Capping R-HSA-159230 Transport of the SLBP Dependant Mature mRNA R-HSA-167158 Formation of the HIV-1 Early Elongation Complex R-HSA-6807505 RNA polymerase II transcribes snRNA genes R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript R-HSA-159227 Transport of the SLBP independent Mature mRNA R-HSA-112382 Formation of RNA Pol II elongation complex R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-77588 SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs R-HSA-109688 Cleavage of Growing Transcript in the Termination Region R-HSA-77595 Processing of Intronless Pre-mRNAs R-HSA-8953854 Metabolism of RNA R-HSA-159234 Transport of Mature mRNAs Derived from Intronless Transcripts R-HSA-9010553 Regulation of expression of SLITs and ROBOs R-HSA-191859 snRNP Assembly R-HSA-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-72165 mRNA Splicing - Minor Pathway R-HSA-72187 mRNA 3'-end processing R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-113418 Formation of the Early Elongation Complex R-HSA-111367 SLBP independent Processing of Histone Pre-mRNAs R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-6803529 FGFR2 alternative splicing R-HSA-8851708 Signaling by FGFR2 IIIa TM R-HSA-167169 HIV Transcription Elongation R-HSA-73857 RNA Polymerase II Transcription R-HSA-75955 RNA Polymerase II Transcription Elongation R-HSA-167172 Transcription of the HIV genome R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript R-HSA-75067 Processing of Capped Intronless Pre-mRNA R-HSA-73856 RNA Polymerase II Transcription Termination R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-376176 Signaling by ROBO receptors R-HSA-194441 Metabolism of non-coding RNA R-HSA-927802 Nonsense-Mediated Decay (NMD) R-HSA-72172 mRNA Splicing R-HSA-5654738 Signaling by FGFR2 R-HSA-1839126 FGFR2 mutant receptor activation R-HSA-74160 Gene expression (Transcription) R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-HSA-422475 Axon guidance R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-190236 Signaling by FGFR R-HSA-5655253 Signaling by FGFR2 in disease R-HSA-162587 HIV Life Cycle R-HSA-1266738 Developmental Biology R-HSA-9006934 Signaling by Receptor Tyrosine Kinases R-HSA-1226099 Signaling by FGFR in disease R-HSA-162906 HIV Infection R-HSA-162582 Signal Transduction R-HSA-5663202 Diseases of signal transduction R-HSA-5663205 Infectious disease R-HSA-1643685 Disease
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
