Human Gene OGA (ENST00000357797.9_6) from GENCODE V47lift37
  Description: [Isoform 1]: Cleaves GlcNAc but not GalNAc from O- glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4- methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta- GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714). (from UniProt O60502)
Gencode Transcript: ENST00000357797.9_6
Gencode Gene: ENSG00000198408.14_15
Transcript (Including UTRs)
   Position: hg19 chr10:103,545,891-103,578,175 Size: 32,285 Total Exon Count: 15 Strand: -
Coding Region
   Position: hg19 chr10:103,546,208-103,577,779 Size: 31,572 Coding Exon Count: 15 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:103,545,891-103,578,175)mRNA (may differ from genome)Protein (849 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblExonPrimerGeneCardsMGIPubMed
UniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: NCOAT_HUMAN
DESCRIPTION: RecName: Full=Bifunctional protein NCOAT; AltName: Full=Meningioma-expressed antigen 5; AltName: Full=Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; Includes: RecName: Full=Protein O-GlcNAcase; EC=3.2.1.169; AltName: Full=Glycoside hydrolase O-GlcNAcase; AltName: Full=Hexosaminidase C; AltName: Full=N-acetyl-beta-D-glucosaminidase; AltName: Full=N-acetyl-beta-glucosaminidase; AltName: Full=O-GlcNAcase; Short=OGA; Includes: RecName: Full=Histone acetyltransferase; Short=HAT; EC=2.3.1.48;
FUNCTION: Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 (By similarity).
CATALYTIC ACTIVITY: [Protein]-3-O-(N-acetyl-D-glucosaminyl)-L- serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine.
CATALYTIC ACTIVITY: [Protein]-3-O-(N-acetyl-D-glucosaminyl)-L- threonine + H(2)O = [protein]-L-threonine + N-acetyl-D- glucosamine.
CATALYTIC ACTIVITY: Acetyl-CoA + [histone] = CoA + acetyl- [histone].
ENZYME REGULATION: Inhibited by N-acetylglucosamine and not N- acetylgalactosamine.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.1 mM for pNP-GLcNAc; Vmax=652 umol/min/mg enzyme with pNP-GLcNAc as substrate; pH dependence: Optimum pH is 5.7-7;
SUBUNIT: Monomer. Binds both acetylated and unacetylated histone H4 tail but acetylation on 'Lys-8' of histone H4 abolishes binding (By similarity).
SUBCELLULAR LOCATION: Isoform 3: Nucleus.
SUBCELLULAR LOCATION: Isoform 1: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitous. Shows highest expression in the brain, placenta and pancreas.
PTM: Proteolytically cleaved by caspase-3.
SEQUENCE CAUTION: Sequence=AAH47877.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=BAA31654.2; Type=Erroneous initiation;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 60.03 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 1741.76 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -176.90396-0.447 Picture PostScript Text
3' UTR -78.20317-0.247 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016181 - Acyl_CoA_acyltransferase
IPR011496 - Beta-N-acetylglucosaminidase
IPR017853 - Glycoside_hydrolase_SF

Pfam Domains:
PF07555 - beta-N-acetylglucosaminidase

SCOP Domains:
51445 - (Trans)glycosidases
55729 - Acyl-CoA N-acyltransferases (Nat)

ModBase Predicted Comparative 3D Structure on O60502
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004415 hyalurononglucosaminidase activity
GO:0016231 beta-N-acetylglucosaminidase activity
GO:0016787 hydrolase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds

Biological Process:
GO:0006044 N-acetylglucosamine metabolic process
GO:0006493 protein O-linked glycosylation
GO:0006516 glycoprotein catabolic process
GO:0006517 protein deglycosylation
GO:0008152 metabolic process

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  AK027008 - Homo sapiens cDNA: FLJ23355 fis, clone HEP14552.
AB014579 - Homo sapiens KIAA0679 mRNA for KIAA0679 protein.
AF036144 - Homo sapiens meningioma-expressed antigen 5 (MEA5) mRNA, complete cds.
BC039583 - Homo sapiens meningioma expressed antigen 5 (hyaluronidase), mRNA (cDNA clone MGC:48750 IMAGE:5558183), complete cds.
BC014201 - Homo sapiens meningioma expressed antigen 5 (hyaluronidase), mRNA (cDNA clone IMAGE:4576364), partial cds.
BC001343 - Homo sapiens, Similar to meningioma expressed antigen 5 (hyaluronidase), clone IMAGE:3462742, mRNA, partial cds.
AK002091 - Homo sapiens cDNA FLJ11229 fis, clone PLACE1008356, highly similar to Homo sapiens meningioma expressed antigen 5 ((MGEA5), mRNA.
KJ902148 - Synthetic construct Homo sapiens clone ccsbBroadEn_11542 MGEA5 gene, encodes complete protein.
AB383953 - Synthetic construct DNA, clone: pF1KSDA0679, Homo sapiens MGEA5 gene for bifunctional protein NCOAT, complete cds, without stop codon, in Flexi system.
JD239257 - Sequence 220281 from Patent EP1572962.
JD228859 - Sequence 209883 from Patent EP1572962.
AK000988 - Homo sapiens cDNA FLJ10126 fis, clone HEMBA1002971.
AF307332 - Homo sapiens meningioma-expressed antigen 5s splice variant mRNA, complete cds.
BC047877 - Homo sapiens meningioma expressed antigen 5 (hyaluronidase), mRNA (cDNA clone IMAGE:5580702), partial cds.
BC032591 - Homo sapiens meningioma expressed antigen 5 (hyaluronidase), mRNA (cDNA clone IMAGE:5503294).
AK302626 - Homo sapiens cDNA FLJ54806 complete cds, highly similar to Homo sapiens meningioma expressed antigen 5 (MGEA5), mRNA.
JD178781 - Sequence 159805 from Patent EP1572962.
JD178319 - Sequence 159343 from Patent EP1572962.
JD438023 - Sequence 419047 from Patent EP1572962.
JD473016 - Sequence 454040 from Patent EP1572962.
JD402664 - Sequence 383688 from Patent EP1572962.
JD486975 - Sequence 467999 from Patent EP1572962.
JD276432 - Sequence 257456 from Patent EP1572962.
JD056763 - Sequence 37787 from Patent EP1572962.
JD186783 - Sequence 167807 from Patent EP1572962.
JD472863 - Sequence 453887 from Patent EP1572962.
JD257077 - Sequence 238101 from Patent EP1572962.
JD183405 - Sequence 164429 from Patent EP1572962.
JD074746 - Sequence 55770 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B7WPB9, BC039583, D3DR79, E9PGF9, ENST00000357797.1, ENST00000357797.2, ENST00000357797.3, ENST00000357797.4, ENST00000357797.5, ENST00000357797.6, ENST00000357797.7, ENST00000357797.8, HEXC, KIAA0679, MEA5, MGEA5, O60502, O75166, OGA , OGA_HUMAN, Q86WV0, Q8IV98, Q9BVA5, Q9HAR0, uc318afu.1, uc318afu.2
UCSC ID: ENST00000357797.9_6
RefSeq Accession: NM_001142434.2
Protein: O60502 (aka NCOAT_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.