Human Gene IER3 (ENST00000259874.6_9) from GENCODE V47lift37
  Description: immediate early response 3 (from RefSeq NM_003897.4)
Gencode Transcript: ENST00000259874.6_9
Gencode Gene: ENSG00000137331.12_11
Transcript (Including UTRs)
   Position: hg19 chr6:30,710,976-30,712,324 Size: 1,349 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr6:30,711,713-30,712,295 Size: 583 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:30,710,976-30,712,324)mRNA (may differ from genome)Protein (156 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IEX1_HUMAN
DESCRIPTION: RecName: Full=Radiation-inducible immediate-early gene IEX-1; AltName: Full=Differentiation-dependent gene 2 protein; Short=Protein DIF-2; AltName: Full=Immediate early protein GLY96; AltName: Full=Immediate early response 3 protein; AltName: Full=PACAP-responsive gene 1 protein; Short=Protein PRG1;
FUNCTION: May play a role in the ERK signaling pathway by inhibiting the dephosphorylation of ERK by phosphatase PP2A- PPP2R5C holoenzyme. Acts also as an ERK downstream effector mediating survival.
SUBUNIT: Interacts with the PPP2R5C-PP2A holoenzyme and ERK kinases; regulates ERK dephosphorylation.
INTERACTION: P49069:CAMLG; NbExp=2; IntAct=EBI-1748945, EBI-1748958;
SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
INDUCTION: By radiation, 12-O-tetradecanoyl phorbol-13 acetate (TPA), okadaic acid and TNF.
PTM: Phosphorylated at Thr-18, Thr-123 and Ser-126 by MAPK1/ERK2 and probably MAPK3/ERK1. Upon phosphorylation by MAPK1/ERK2 and MAPK3/ERK1, acquires the ability to inhibit cell death induced by various stimuli.
PTM: Glycosylated.
SIMILARITY: Belongs to the IER3 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: IER3
Diseases sorted by gene-association score: kallmann syndrome (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 80.86 RPKM in Vagina
Total median expression: 1225.35 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -3.4029-0.117 Picture PostScript Text
3' UTR -250.40737-0.340 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR024829 - IEX-1

ModBase Predicted Comparative 3D Structure on P46695
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0006915 apoptotic process
GO:0009653 anatomical structure morphogenesis
GO:0010941 regulation of cell death
GO:0014066 regulation of phosphatidylinositol 3-kinase signaling
GO:0043066 negative regulation of apoptotic process
GO:2001020 regulation of response to DNA damage stimulus

Cellular Component:
GO:0005634 nucleus
GO:0005829 cytosol
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  LF210806 - JP 2014500723-A/18309: Polycomb-Associated Non-Coding RNAs.
BC000844 - Homo sapiens immediate early response 3, mRNA (cDNA clone MGC:5118 IMAGE:3457670), complete cds.
AJ227914 - Homo sapiens partial mRNA; ID YG40-2.
Y14551 - Homo sapiens mRNA for DIF-2 protein.
S81914 - IEX-1=radiation-inducible immediate-early gene [human, placenta, mRNA Partial, 1223 nt].
BC005080 - Homo sapiens immediate early response 3, mRNA (cDNA clone MGC:13037 IMAGE:3617088), complete cds.
AF039067 - Homo sapiens anti-death protein (IEX-1L) mRNA, complete cds.
LF214046 - JP 2014500723-A/21549: Polycomb-Associated Non-Coding RNAs.
AK222588 - Homo sapiens mRNA for immediate early response 3 isoform short variant, clone: CAS03656.
LF341309 - JP 2014500723-A/148812: Polycomb-Associated Non-Coding RNAs.
JD139077 - Sequence 120101 from Patent EP1572962.
JD139078 - Sequence 120102 from Patent EP1572962.
JD078921 - Sequence 59945 from Patent EP1572962.
JD081703 - Sequence 62727 from Patent EP1572962.
JD046403 - Sequence 27427 from Patent EP1572962.
JD094027 - Sequence 75051 from Patent EP1572962.
JD469914 - Sequence 450938 from Patent EP1572962.
LF341308 - JP 2014500723-A/148811: Polycomb-Associated Non-Coding RNAs.
JD244029 - Sequence 225053 from Patent EP1572962.
JD281197 - Sequence 262221 from Patent EP1572962.
JD127887 - Sequence 108911 from Patent EP1572962.
JD127888 - Sequence 108912 from Patent EP1572962.
JD329470 - Sequence 310494 from Patent EP1572962.
JD329471 - Sequence 310495 from Patent EP1572962.
JD123040 - Sequence 104064 from Patent EP1572962.
AF083421 - Homo sapiens radiation-inducible immediate early response gene IEX1 (IEX1) mRNA, complete cds.
AK314162 - Homo sapiens cDNA, FLJ94872, Homo sapiens immediate early response 3 (IER3), transcript variantshort, mRNA.
BT006703 - Homo sapiens immediate early response 3 mRNA, complete cds.
KJ897922 - Synthetic construct Homo sapiens clone ccsbBroadEn_07316 IER3 gene, encodes complete protein.
AB591006 - Synthetic construct DNA, clone: pFN21AE1642, Homo sapiens IER3 gene for immediate early response 3, without stop codon, in Flexi system.
LF341307 - JP 2014500723-A/148810: Polycomb-Associated Non-Coding RNAs.
JD025526 - Sequence 6550 from Patent EP1572962.
MA449623 - JP 2018138019-A/21549: Polycomb-Associated Non-Coding RNAs.
MA446383 - JP 2018138019-A/18309: Polycomb-Associated Non-Coding RNAs.
MA576886 - JP 2018138019-A/148812: Polycomb-Associated Non-Coding RNAs.
MA576885 - JP 2018138019-A/148811: Polycomb-Associated Non-Coding RNAs.
MA576884 - JP 2018138019-A/148810: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P46695 (Reactome details) participates in the following event(s):

R-HSA-6811472 IER3 recruits MAPKs to PP2A-B56-beta,gamma
R-HSA-6811454 MAPKs phosphorylate PP2A
R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-199418 Negative regulation of the PI3K/AKT network
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: DIF2, ENST00000259874.1, ENST00000259874.2, ENST00000259874.3, ENST00000259874.4, ENST00000259874.5, IEX1, IEX1_HUMAN, NM_003897, P46695, PRG1, Q5SU30, Q92691, Q93044, uc317ghz.1, uc317ghz.2
UCSC ID: ENST00000259874.6_9
RefSeq Accession: NM_003897.4
Protein: P46695 (aka IEX1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.