Human Gene EIF5 (ENST00000216554.8_4) from GENCODE V47lift37
  Description: eukaryotic translation initiation factor 5, transcript variant 1 (from RefSeq NM_001969.5)
Gencode Transcript: ENST00000216554.8_4
Gencode Gene: ENSG00000100664.11_11
Transcript (Including UTRs)
   Position: hg19 chr14:103,800,574-103,811,362 Size: 10,789 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg19 chr14:103,802,198-103,807,389 Size: 5,192 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:103,800,574-103,811,362)mRNA (may differ from genome)Protein (431 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IF5_HUMAN
DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 5; Short=eIF-5;
FUNCTION: Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
SIMILARITY: Belongs to the eIF-2-beta/eIF-5 family.
SIMILARITY: Contains 1 W2 domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: EIF5
Diseases sorted by gene-association score: leukoencephalopathy with vanishing white matter (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 38.54 RPKM in Liver
Total median expression: 1482.68 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -139.50441-0.316 Picture PostScript Text
3' UTR -1100.103973-0.277 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016024 - ARM-type_fold
IPR016021 - MIF4-like_typ_1/2/3
IPR002735 - Transl_init_fac_IF2/IF5
IPR016189 - Transl_init_fac_IF2/IF5_N
IPR016190 - Transl_init_fac_IF2/IF5_Zn-bd
IPR003307 - W2_domain

Pfam Domains:
PF01873 - Domain found in IF2B/IF5
PF02020 - eIF4-gamma/eIF5/eIF2-epsilon

SCOP Domains:
48371 - ARM repeat
101447 - Formin homology 2 domain (FH2 domain)
100966 - Translation initiation factor 2 beta, aIF2beta, N-terminal domain
75689 - Zinc-binding domain of translation initiation factor 2 beta

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2E9H - NMR MuPIT 2G2K - NMR MuPIT 2IU1 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P55010
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003723 RNA binding
GO:0003743 translation initiation factor activity
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0008135 translation factor activity, RNA binding
GO:0045296 cadherin binding
GO:0071074 eukaryotic initiation factor eIF2 binding

Biological Process:
GO:0001731 formation of translation preinitiation complex
GO:0006412 translation
GO:0006413 translational initiation
GO:0006446 regulation of translational initiation
GO:0090630 activation of GTPase activity

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AK026933 - Homo sapiens cDNA: FLJ23280 fis, clone HEP07194.
BX537367 - Homo sapiens mRNA; cDNA DKFZp686K0727 (from clone DKFZp686K0727); complete cds.
CR627002 - Homo sapiens mRNA; cDNA DKFZp686M15120 (from clone DKFZp686M15120).
BC006275 - Homo sapiens cDNA clone IMAGE:3952928, **** WARNING: chimeric clone ****.
BC041083 - Homo sapiens, clone IMAGE:5094332, mRNA.
JD560123 - Sequence 541147 from Patent EP1572962.
AL080102 - Homo sapiens mRNA; cDNA DKFZp564N1916 (from clone DKFZp564N1916).
BC107884 - Homo sapiens eukaryotic translation initiation factor 5, mRNA (cDNA clone IMAGE:6672764), partial cds.
U49436 - Human translation initiation factor 5 (eIF5) mRNA, complete cds.
BC000106 - Homo sapiens, Similar to eukaryotic translation initiation factor 5, clone IMAGE:3504816, mRNA.
JD227594 - Sequence 208618 from Patent EP1572962.
BC007728 - Homo sapiens eukaryotic translation initiation factor 5, mRNA (cDNA clone MGC:12671 IMAGE:4303500), complete cds.
JD462463 - Sequence 443487 from Patent EP1572962.
JD140828 - Sequence 121852 from Patent EP1572962.
JD351756 - Sequence 332780 from Patent EP1572962.
BC032866 - Homo sapiens eukaryotic translation initiation factor 5, mRNA (cDNA clone MGC:33829 IMAGE:5261866), complete cds.
BC015630 - Homo sapiens eukaryotic translation initiation factor 5, mRNA (cDNA clone IMAGE:3542638), partial cds.
JD083370 - Sequence 64394 from Patent EP1572962.
JD227971 - Sequence 208995 from Patent EP1572962.
JD553311 - Sequence 534335 from Patent EP1572962.
JD165191 - Sequence 146215 from Patent EP1572962.
JD379131 - Sequence 360155 from Patent EP1572962.
JD188503 - Sequence 169527 from Patent EP1572962.
CU689572 - Synthetic construct Homo sapiens gateway clone IMAGE:100019363 5' read EIF5 mRNA.
CU675779 - Synthetic construct Homo sapiens gateway clone IMAGE:100017253 5' read EIF5 mRNA.
AB528356 - Synthetic construct DNA, clone: pF1KB5142, Homo sapiens EIF5 gene for eukaryotic translation initiation factor 5, without stop codon, in Flexi system.
DQ893482 - Synthetic construct clone IMAGE:100006112; FLH193882.01X; RZPDo839D0179D eukaryotic translation initiation factor 5 (EIF5) gene, encodes complete protein.
EU832284 - Synthetic construct Homo sapiens clone HAIB:100067313; DKFZo008A1127 eukaryotic translation initiation factor 5 protein (EIF5) gene, encodes complete protein.
EU832369 - Synthetic construct Homo sapiens clone HAIB:100067398; DKFZo004A1128 eukaryotic translation initiation factor 5 protein (EIF5) gene, encodes complete protein.
HQ448292 - Synthetic construct Homo sapiens clone IMAGE:100071696; CCSB004989_02 eukaryotic translation initiation factor 5 (EIF5) gene, encodes complete protein.
KJ896757 - Synthetic construct Homo sapiens clone ccsbBroadEn_06151 EIF5 gene, encodes complete protein.
KJ896758 - Synthetic construct Homo sapiens clone ccsbBroadEn_06152 EIF5 gene, encodes complete protein.
CR456712 - Homo sapiens full open reading frame cDNA clone RZPDo834G0811D for gene EIF5, eukaryotic translation initiation factor 5; complete cds, incl. stopcodon.
AK293712 - Homo sapiens cDNA FLJ61101 complete cds, highly similar to Eukaryotic translation initiation factor 5.
JD350437 - Sequence 331461 from Patent EP1572962.
JD189977 - Sequence 171001 from Patent EP1572962.
JD038622 - Sequence 19646 from Patent EP1572962.
JD506398 - Sequence 487422 from Patent EP1572962.
JD237159 - Sequence 218183 from Patent EP1572962.
JD151324 - Sequence 132348 from Patent EP1572962.
JD428904 - Sequence 409928 from Patent EP1572962.
JD101586 - Sequence 82610 from Patent EP1572962.
JD306012 - Sequence 287036 from Patent EP1572962.
JD565906 - Sequence 546930 from Patent EP1572962.
JD209189 - Sequence 190213 from Patent EP1572962.
JD022785 - Sequence 3809 from Patent EP1572962.
JD050171 - Sequence 31195 from Patent EP1572962.
JD034975 - Sequence 15999 from Patent EP1572962.
JD540517 - Sequence 521541 from Patent EP1572962.
AK027240 - Homo sapiens cDNA: FLJ23587 fis, clone LNG14395.
AK074470 - Homo sapiens cDNA FLJ23890 fis, clone LNG14419.
JD287228 - Sequence 268252 from Patent EP1572962.
DQ573635 - Homo sapiens piRNA piR-41747, complete sequence.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_eif2Pathway - Regulation of eIF2
h_eifPathway - Eukaryotic protein translation

Reactome (by CSHL, EBI, and GO)

Protein P55010 (Reactome details) participates in the following event(s):

R-HSA-72619 eIF2:GTP is hydrolyzed, eIFs are released
R-HSA-72697 Start codon recognition
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-72737 Cap-dependent Translation Initiation
R-HSA-72613 Eukaryotic Translation Initiation
R-HSA-72766 Translation
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000216554.1, ENST00000216554.2, ENST00000216554.3, ENST00000216554.4, ENST00000216554.5, ENST00000216554.6, ENST00000216554.7, IF5_HUMAN, NM_001969, P55010, Q53XB3, Q9H5N2, Q9UG48, uc317crn.1, uc317crn.2
UCSC ID: ENST00000216554.8_4
RefSeq Accession: NM_001969.5
Protein: P55010 (aka IF5_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.