ID:EIF3E_HUMAN DESCRIPTION: RecName: Full=Eukaryotic translation initiation factor 3 subunit E; Short=eIF3e; AltName: Full=Eukaryotic translation initiation factor 3 subunit 6; AltName: Full=Viral integration site protein INT-6 homolog; AltName: Full=eIF-3 p48; FUNCTION: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense- mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. SUBUNIT: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with COPS3, COPS6, COPS7 (COPS7A or COPS7B), EIF4G1, EPAS1, MCM7, NCBP1, PSMC6, TRIM27 and UPF2. Interacts with the HTLV-1 protein Tax-1. Interacts with IFIT1 and IFIT2. INTERACTION: Q99814:EPAS1; NbExp=10; IntAct=EBI-347740, EBI-447470; Q9Q2G4:ORF (xeno); NbExp=5; IntAct=EBI-347740, EBI-6248094; P14373:TRIM27; NbExp=7; IntAct=EBI-347740, EBI-719493; SUBCELLULAR LOCATION: Cytoplasm. Nucleus, PML body. TISSUE SPECIFICITY: Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). MASS SPECTROMETRY: Mass=52131.8; Method=Unknown; Range=1-445; Source=PubMed:17322308; MASS SPECTROMETRY: Mass=52133.4; Mass_error=0.2; Method=MALDI; Range=1-445; Source=PubMed:18599441; SIMILARITY: Belongs to the eIF-3 subunit E family. SIMILARITY: Contains 1 PCI domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P60228
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
KJ905813 - Synthetic construct Homo sapiens clone ccsbBroadEn_15483 EIF3E gene, encodes complete protein. KJ891484 - Synthetic construct Homo sapiens clone ccsbBroadEn_00878 EIF3E gene, encodes complete protein. KJ905812 - Synthetic construct Homo sapiens clone ccsbBroadEn_15482 EIF3E gene, encodes complete protein. KJ905814 - Synthetic construct Homo sapiens clone ccsbBroadEn_15484 EIF3E gene, encodes complete protein. AL355697 - Homo sapiens EST from clone 1695848, 5' end. U85947 - Homo sapiens Int-6 mRNA, complete cds. BC021679 - Homo sapiens eukaryotic translation initiation factor 3, subunit E, mRNA (cDNA clone MGC:23768 IMAGE:4132737), complete cds. U94175 - Homo sapiens mammary tumor-associated protein INT6 (INT6) mRNA, complete cds. U62962 - Human Int-6 mRNA, complete cds. U54562 - Human translation initiation factor eIF3 p48 subunit (Int-6) mRNA, complete cds. BC017887 - Homo sapiens eukaryotic translation initiation factor 3, subunit E, mRNA (cDNA clone MGC:22770 IMAGE:4685684), complete cds. BC016706 - Homo sapiens eukaryotic translation initiation factor 3, subunit E, mRNA (cDNA clone MGC:23718 IMAGE:4096434), complete cds. BC005944 - Homo sapiens eukaryotic translation initiation factor 3, subunit 6 48kDa, mRNA (cDNA clone IMAGE:4106529). AK124178 - Homo sapiens cDNA FLJ42184 fis, clone THYMU2031994, highly similar to Eukaryotic translation initiation factor 3 subunit 6. BC000734 - Homo sapiens eukaryotic translation initiation factor 3, subunit E, mRNA (cDNA clone MGC:2060 IMAGE:2959855), complete cds. BC008419 - Homo sapiens eukaryotic translation initiation factor 3, subunit E, mRNA (cDNA clone MGC:14568 IMAGE:4080571), complete cds. AK313186 - Homo sapiens cDNA, FLJ93685, highly similar to Homo sapiens eukaryotic translation initiation factor 3, subunit 6 48kDa (EIF3S6), mRNA. DQ892278 - Synthetic construct clone IMAGE:100004908; FLH184802.01X; RZPDo839C01146D eukaryotic translation initiation factor 3, subunit 6 48kDa (EIF3S6) gene, encodes complete protein. DQ895478 - Synthetic construct Homo sapiens clone IMAGE:100009938; FLH184798.01L; RZPDo839C01145D eukaryotic translation initiation factor 3, subunit 6 48kDa (EIF3S6) gene, encodes complete protein. AB527268 - Synthetic construct DNA, clone: pF1KB5352, Homo sapiens EIF3E gene for eukaryotic translation initiation factor 3, subunit E, without stop codon, in Flexi system. CR542275 - Homo sapiens full open reading frame cDNA clone RZPDo834H0726D for gene EIF3S6, eukaryotic translation initiation factor 3, subunit 6 48kDa; complete cds, without stopcodon. CR457029 - Homo sapiens full open reading frame cDNA clone RZPDo834C0118D for gene EIF3S6, eukaryotic translation initiation factor 3, subunit 6 48kDa; complete cds, incl. stopcodon. CU674376 - Synthetic construct Homo sapiens gateway clone IMAGE:100017284 5' read EIF3E mRNA. CU677689 - Synthetic construct Homo sapiens gateway clone IMAGE:100017286 5' read EIF3E mRNA. CU678957 - Synthetic construct Homo sapiens gateway clone IMAGE:100019434 5' read EIF3E mRNA. KC333871 - Homo sapiens eukaryotic translation initiation factor 3 subunit E isoform 2 transcript variant (EIF3E) mRNA, complete cds. LQ302625 - Sequence 19 from Patent WO2016063192. AK310902 - Homo sapiens cDNA, FLJ17944. JD203964 - Sequence 184988 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P60228 (Reactome details) participates in the following event(s):
R-HSA-72676 eIF3 and eIF1A bind to the 40S subunit R-HSA-72619 eIF2:GTP is hydrolyzed, eIFs are released R-HSA-72691 Formation of the 43S pre-initiation complex R-HSA-156808 Formation of translation initiation complexes yielding circularized Ceruloplasmin mRNA in a 'closed-loop' conformation R-HSA-157849 Formation of translation initiation complexes containing mRNA that does not circularize R-HSA-72621 Ribosomal scanning R-HSA-72697 Start codon recognition R-HSA-156823 Association of phospho-L13a with GAIT element of Ceruloplasmin mRNA R-HSA-72689 Formation of a pool of free 40S subunits R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72649 Translation initiation complex formation R-HSA-72737 Cap-dependent Translation Initiation R-HSA-72702 Ribosomal scanning and start codon recognition R-HSA-156827 L13a-mediated translation R-HSA-72662 Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-HSA-72613 Eukaryotic Translation Initiation R-HSA-72766 Translation R-HSA-392499 Metabolism of proteins
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
