ID:EHMT2_HUMAN DESCRIPTION: RecName: Full=Histone-lysine N-methyltransferase EHMT2; EC=2.1.1.-; EC=2.1.1.43; AltName: Full=Euchromatic histone-lysine N-methyltransferase 2; AltName: Full=HLA-B-associated transcript 8; AltName: Full=Histone H3-K9 methyltransferase 3; Short=H3-K9-HMTase 3; AltName: Full=Lysine N-methyltransferase 1C; AltName: Full=Protein G9a; FUNCTION: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. CATALYTIC ACTIVITY: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]. SUBUNIT: Heterodimer; heterodimerizes with EHMT1/GLP. Interacts with GFI1B and WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. Interacts with UHRF1. INTERACTION: Q99684:GFI1; NbExp=2; IntAct=EBI-744366, EBI-949368; Q07120:Gfi1 (xeno); NbExp=3; IntAct=EBI-744366, EBI-4289236; Q13547:HDAC1; NbExp=2; IntAct=EBI-744366, EBI-301834; Q9NQX1:PRDM5; NbExp=3; IntAct=EBI-744366, EBI-4292031; Q5JSZ5:PRRC2B; NbExp=2; IntAct=EBI-744366, EBI-744891; SUBCELLULAR LOCATION: Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin. TISSUE SPECIFICITY: Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. DOMAIN: The SET domain mediates interaction with WIZ. DOMAIN: The ANK repeats bind H3K9me1 and H3K9me2. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. PTM: Methylated at Lys-185; automethylated. SIMILARITY: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. SIMILARITY: Contains 7 ANK repeats. SIMILARITY: Contains 1 post-SET domain. SIMILARITY: Contains 1 pre-SET domain. SIMILARITY: Contains 1 SET domain. CAUTION: While NG36 and G9a were originally thought to derive from 2 separate genes, all G9A transcripts also contain the in frame coding sequence of NG36 (PubMed:11707778). CAUTION: It is uncertain whether Met-1 or Met-21 is the initiator methionine. SEQUENCE CAUTION: Sequence=AAD21811.1; Type=Erroneous gene model prediction; Sequence=AAD21812.1; Type=Erroneous gene model prediction; Sequence=AAH02686.2; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=AAH09351.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=AAH18718.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAH20970.2; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAB63294.1; Type=Erroneous gene model prediction; Sequence=BAB63295.1; Type=Erroneous gene model prediction; Sequence=CAA49491.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q96KQ7
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
