ID:E2F1_HUMAN DESCRIPTION: RecName: Full=Transcription factor E2F1; Short=E2F-1; AltName: Full=PBR3; AltName: Full=Retinoblastoma-associated protein 1; Short=RBAP-1; AltName: Full=Retinoblastoma-binding protein 3; Short=RBBP-3; AltName: Full=pRB-binding protein E2F-1; FUNCTION: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. ENZYME REGULATION: BIRC2/c-IAP1 stimulates its transcriptional activity. SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T- antigen and HPV E7, are capable of sequestering RB1, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity. Interacts with BIRC2/c-IAP1 (via BIR domains). Interacts with human cytomegalovirus/HHV-5 protein UL123. INTERACTION: Q92466:DDB2; NbExp=2; IntAct=EBI-448924, EBI-1176171; Q9Y618:NCOR2; NbExp=2; IntAct=EBI-448924, EBI-80830; P09874:PARP1; NbExp=2; IntAct=EBI-448924, EBI-355676; P06400:RB1; NbExp=10; IntAct=EBI-448924, EBI-491274; Q96EB6:SIRT1; NbExp=3; IntAct=EBI-448924, EBI-1802965; Q923E4:Sirt1 (xeno); NbExp=3; IntAct=EBI-448924, EBI-1802585; P08047:SP1; NbExp=2; IntAct=EBI-448924, EBI-298336; P42224:STAT1; NbExp=2; IntAct=EBI-448924, EBI-1057697; O95361:TRIM16; NbExp=2; IntAct=EBI-448924, EBI-727384; SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis. PTM: Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. SIMILARITY: Belongs to the E2F/DP family. SEQUENCE CAUTION: Sequence=AAB24289.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/E2F1ID40382ch20q11.html"; WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/e2f1/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q01094
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding GO:0001047 core promoter binding GO:0001227 transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding GO:0003677 DNA binding GO:0003700 transcription factor activity, sequence-specific DNA binding GO:0005515 protein binding GO:0008134 transcription factor binding GO:0019901 protein kinase binding GO:0043565 sequence-specific DNA binding GO:0044212 transcription regulatory region DNA binding GO:0046983 protein dimerization activity
Biological Process: GO:0000077 DNA damage checkpoint GO:0000083 regulation of transcription involved in G1/S transition of mitotic cell cycle GO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006915 apoptotic process GO:0006977 DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0007049 cell cycle GO:0007283 spermatogenesis GO:0008630 intrinsic apoptotic signaling pathway in response to DNA damage GO:0010628 positive regulation of gene expression GO:0030900 forebrain development GO:0043065 positive regulation of apoptotic process GO:0043276 anoikis GO:0043392 negative regulation of DNA binding GO:0045599 negative regulation of fat cell differentiation GO:0045892 negative regulation of transcription, DNA-templated GO:0045893 positive regulation of transcription, DNA-templated GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0048146 positive regulation of fibroblast proliferation GO:0048255 mRNA stabilization GO:0051726 regulation of cell cycle GO:0070317 negative regulation of G0 to G1 transition GO:0070345 negative regulation of fat cell proliferation GO:0071398 cellular response to fatty acid GO:0071456 cellular response to hypoxia GO:0071466 cellular response to xenobiotic stimulus GO:0071930 negative regulation of transcription involved in G1/S transition of mitotic cell cycle GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator GO:1900740 positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway GO:1990086 lens fiber cell apoptotic process GO:1990090 cellular response to nerve growth factor stimulus GO:2000045 regulation of G1/S transition of mitotic cell cycle
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
