ID:DDX5_HUMAN DESCRIPTION: RecName: Full=Probable ATP-dependent RNA helicase DDX5; EC=3.6.4.13; AltName: Full=DEAD box protein 5; AltName: Full=RNA helicase p68; FUNCTION: Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. SUBUNIT: Interacts with BRDT (By similarity). Identified in the spliceosome C complex. Interacts with RBM4; the interaction occurs in a RNA-independent manner. Interacts with EIF2C1 and EIF2C2. Interacts with ESR1; the interaction is enhanced by phosphorylation of ESR1 AF-1 domain. Interacts with AR, NCOA1, NCOA2, NCOA3, EP300, CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self- associates. Interacts with DDX17. INTERACTION: Q12873:CHD3; NbExp=4; IntAct=EBI-351962, EBI-523590; P45481:Crebbp (xeno); NbExp=3; IntAct=EBI-351962, EBI-296306; Q09472:EP300; NbExp=4; IntAct=EBI-351962, EBI-447295; P03372:ESR1; NbExp=8; IntAct=EBI-351962, EBI-78473; P22087:FBL; NbExp=6; IntAct=EBI-351962, EBI-358318; Q13547:HDAC1; NbExp=4; IntAct=EBI-351962, EBI-301834; O95983:MBD3; NbExp=4; IntAct=EBI-351962, EBI-1783068; P10085:Myod1 (xeno); NbExp=3; IntAct=EBI-351962, EBI-4405734; P24928:POLR2A; NbExp=3; IntAct=EBI-351962, EBI-295301; Q08775-3:Runx2 (xeno); NbExp=2; IntAct=EBI-351962, EBI-6119991; P04637:TP53; NbExp=3; IntAct=EBI-351962, EBI-366083; P04637-1:TP53; NbExp=2; IntAct=EBI-351962, EBI-3895849; P04637-7:TP53; NbExp=2; IntAct=EBI-351962, EBI-3895873; SUBCELLULAR LOCATION: Nucleus, nucleolus. PTM: Arg-502 is dimethylated, probably to asymmetric dimethylarginine. PTM: Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination. PTM: Polyubiquitinated, leading to proteasomal degradation. SIMILARITY: Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. SIMILARITY: Contains 1 helicase ATP-binding domain. SIMILARITY: Contains 1 helicase C-terminal domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P17844
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0000380 alternative mRNA splicing, via spliceosome GO:0000381 regulation of alternative mRNA splicing, via spliceosome GO:0000398 mRNA splicing, via spliceosome GO:0000956 nuclear-transcribed mRNA catabolic process GO:0001837 epithelial to mesenchymal transition GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006357 regulation of transcription from RNA polymerase II promoter GO:0006397 mRNA processing GO:0008380 RNA splicing GO:0009299 mRNA transcription GO:0010501 RNA secondary structure unwinding GO:0016049 cell growth GO:0030509 BMP signaling pathway GO:0030520 intracellular estrogen receptor signaling pathway GO:0030521 androgen receptor signaling pathway GO:0043517 positive regulation of DNA damage response, signal transduction by p53 class mediator GO:0045069 regulation of viral genome replication GO:0045445 myoblast differentiation GO:0045667 regulation of osteoblast differentiation GO:0048511 rhythmic process GO:0060765 regulation of androgen receptor signaling pathway GO:0061614 pri-miRNA transcription from RNA polymerase II promoter GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator GO:1903800 positive regulation of production of miRNAs involved in gene silencing by miRNA GO:2001014 regulation of skeletal muscle cell differentiation GO:1902893 regulation of pri-miRNA transcription from RNA polymerase II promoter
LF213995 - JP 2014500723-A/21498: Polycomb-Associated Non-Coding RNAs. AL133585 - Homo sapiens mRNA; cDNA DKFZp434E109 (from clone DKFZp434E109). AB209919 - Homo sapiens mRNA for Hypothetical protein DKFZp686J01190 variant protein. BC016027 - Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 5, mRNA (cDNA clone MGC:1516 IMAGE:3528578), complete cds. X15729 - Human mRNA for nuclear p68 protein. LP895359 - Sequence 223 from Patent EP3253886. X52104 - Human mRNA for p68 protein. AK055995 - Homo sapiens cDNA FLJ31433 fis, clone NT2NE2000575, highly similar to PROBABLE RNA-DEPENDENT HELICASE P68. AK297192 - Homo sapiens cDNA FLJ59339 complete cds, highly similar to Probable ATP-dependent RNA helicase DDX5 (EC 3.6.1.-). BX571764 - Homo sapiens mRNA; cDNA DKFZp686J01190 (from clone DKFZp686J01190). AK297905 - Homo sapiens cDNA FLJ59357 complete cds, highly similar to Probable ATP-dependent RNA helicase DDX5 (EC 3.6.1.-). JD237659 - Sequence 218683 from Patent EP1572962. AK297753 - Homo sapiens cDNA FLJ53366 complete cds, highly similar to Probable ATP-dependent RNA helicase DDX5 (EC 3.6.1.-). AK310318 - Homo sapiens cDNA, FLJ17360. DQ890687 - Synthetic construct clone IMAGE:100003317; FLH165134.01X; RZPDo839G05158D DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5) gene, encodes complete protein. AB451257 - Homo sapiens DDX5 mRNA for ATP-dependent RNA helicase DDX5, complete cds, clone: FLJ08070AAAN. BT006943 - Homo sapiens DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa) mRNA, complete cds. DQ893774 - Synthetic construct Homo sapiens clone IMAGE:100008234; FLH165130.01L; RZPDo839G05157D DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5) gene, encodes complete protein. AB451382 - Homo sapiens DDX5 mRNA for ATP-dependent RNA helicase DDX5, partial cds, clone: FLJ08070AAAF. AB384902 - Synthetic construct DNA, clone: pF1KB4122, Homo sapiens DDX5 gene for ATP-dependent RNA helicase DDX5, complete cds, without stop codon, in Flexi system. JD019308 - Sequence 332 from Patent EP1572962. JD030780 - Sequence 11804 from Patent EP1572962. DL492053 - Novel nucleic acids. JD022026 - Sequence 3050 from Patent EP1572962. JD020172 - Sequence 1196 from Patent EP1572962. JD021250 - Sequence 2274 from Patent EP1572962. JD027775 - Sequence 8799 from Patent EP1572962. JD028556 - Sequence 9580 from Patent EP1572962. JD035561 - Sequence 16585 from Patent EP1572962. JD035431 - Sequence 16455 from Patent EP1572962. JD020160 - Sequence 1184 from Patent EP1572962. JD019006 - Sequence 30 from Patent EP1572962. JD021339 - Sequence 2363 from Patent EP1572962. JD035257 - Sequence 16281 from Patent EP1572962. JD433220 - Sequence 414244 from Patent EP1572962. MA449572 - JP 2018138019-A/21498: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_akap95Pathway - AKAP95 role in mitosis and chromosome dynamics
Reactome (by CSHL, EBI, and GO)
Protein P17844 (Reactome details) participates in the following event(s):
R-HSA-72107 Formation of the Spliceosomal E complex R-HSA-9009536 DDX5 binds ESR1:estrogen:TFF1 gene promoter R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-9009533 TBP and TFIIA bind TATA box on ESR1:estrogen bound TFF1 gene promoter R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-156661 Formation of Exon Junction Complex R-HSA-9009526 CARM1 binds TBP:TFIIA:DDX5:ESR1:estrogen:TFF1 gene R-HSA-9023861 PRMT1 binds TBP:TFIIA:DDX5:ESR1:estrogen:TFF1 gene R-HSA-9023860 Histone acetyltransferases are recruited to the TFF1 gene R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-9018519 Estrogen-dependent gene expression R-HSA-72172 mRNA Splicing R-HSA-8939211 ESR-mediated signaling R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-9006931 Signaling by Nuclear Receptors R-HSA-8953854 Metabolism of RNA R-HSA-162582 Signal Transduction
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
