Human Gene CNR2 (ENST00000374472.5_7) from GENCODE V47lift37
  Description: cannabinoid receptor 2 (from RefSeq NM_001841.3)
Gencode Transcript: ENST00000374472.5_7
Gencode Gene: ENSG00000188822.8_10
Transcript (Including UTRs)
   Position: hg19 chr1:24,197,005-24,239,852 Size: 42,848 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr1:24,201,025-24,202,107 Size: 1,083 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:24,197,005-24,239,852)mRNA (may differ from genome)Protein (360 aa)
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-  Comments and Description Text from UniProtKB
  ID: CNR2_HUMAN
DESCRIPTION: RecName: Full=Cannabinoid receptor 2; Short=CB-2; Short=CB2; Short=hCB2; AltName: Full=CX5;
FUNCTION: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cell projection, dendrite (By similarity). Perikaryon (By similarity). Note=Localizes to apical dendrite of pyramidal neurons (By similarity).
TISSUE SPECIFICITY: Preferentially expressed in cells of the immune system with higher expression in B-cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
PTM: Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
SIMILARITY: Belongs to the G-protein coupled receptor 1 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CNR2
Diseases sorted by gene-association score: cannabis abuse (9), glycerol kinase deficiency (6), chromosome xp21 deletion syndrome (6), alternating hemiplegia of childhood (4), multiple sclerosis, disease progression, modifier of (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 6.96 RPKM in Spleen
Total median expression: 13.47 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -47.30162-0.292 Picture PostScript Text
3' UTR -1503.604020-0.374 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000276 - 7TM_GPCR_Rhodpsn
IPR001551 - Canbinoid_rcpt_2
IPR002230 - Cnbnoid_rcpt
IPR017452 - GPCR_Rhodpsn_supfam

Pfam Domains:
PF00001 - 7 transmembrane receptor (rhodopsin family)
PF10320 - Serpentine type 7TM GPCR chemoreceptor Srsx

SCOP Domains:
81321 - Family A G protein-coupled receptor-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2KI9 - NMR


ModBase Predicted Comparative 3D Structure on P34972
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0004930 G-protein coupled receptor activity
GO:0004949 cannabinoid receptor activity

Biological Process:
GO:0001975 response to amphetamine
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007165 signal transduction
GO:0007186 G-protein coupled receptor signaling pathway
GO:0007187 G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
GO:0019233 sensory perception of pain
GO:0030595 leukocyte chemotaxis
GO:0032229 negative regulation of synaptic transmission, GABAergic
GO:0032496 response to lipopolysaccharide
GO:0033004 negative regulation of mast cell activation
GO:0038171 cannabinoid signaling pathway
GO:0045759 negative regulation of action potential
GO:0050728 negative regulation of inflammatory response
GO:0051001 negative regulation of nitric-oxide synthase activity

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030425 dendrite
GO:0031234 extrinsic component of cytoplasmic side of plasma membrane
GO:0042995 cell projection
GO:0043005 neuron projection
GO:0043025 neuronal cell body
GO:0043204 perikaryon


-  Descriptions from all associated GenBank mRNAs
  EU517121 - Homo sapiens testis-dominant CNR2 isoform CB2 (CNR2) mRNA, complete cds.
JD095257 - Sequence 76281 from Patent EP1572962.
JD463081 - Sequence 444105 from Patent EP1572962.
JD337555 - Sequence 318579 from Patent EP1572962.
JD533810 - Sequence 514834 from Patent EP1572962.
JD371263 - Sequence 352287 from Patent EP1572962.
JD493391 - Sequence 474415 from Patent EP1572962.
X74328 - H.sapiens mRNA for CB2 (peripheral) cannabinoid receptor.
JD183281 - Sequence 164305 from Patent EP1572962.
BC069722 - Homo sapiens cannabinoid receptor 2 (macrophage), mRNA (cDNA clone MGC:97279 IMAGE:7262528), complete cds.
JD210179 - Sequence 191203 from Patent EP1572962.
JD479242 - Sequence 460266 from Patent EP1572962.
JD460751 - Sequence 441775 from Patent EP1572962.
JD460750 - Sequence 441774 from Patent EP1572962.
JD379484 - Sequence 360508 from Patent EP1572962.
JD038595 - Sequence 19619 from Patent EP1572962.
JD163127 - Sequence 144151 from Patent EP1572962.
JD342594 - Sequence 323618 from Patent EP1572962.
JD401463 - Sequence 382487 from Patent EP1572962.
JD096684 - Sequence 77708 from Patent EP1572962.
JD076208 - Sequence 57232 from Patent EP1572962.
JD468433 - Sequence 449457 from Patent EP1572962.
JD181299 - Sequence 162323 from Patent EP1572962.
JD429329 - Sequence 410353 from Patent EP1572962.
JD325663 - Sequence 306687 from Patent EP1572962.
JD287481 - Sequence 268505 from Patent EP1572962.
AJ430063 - Homo sapiens mRNA for peripheral cannabinoid receptor CB2 (CNR2 gene).
BC074767 - Homo sapiens cannabinoid receptor 2 (macrophage), mRNA (cDNA clone MGC:103805 IMAGE:30915186), complete cds.
AM156854 - Homo sapiens partial mRNA for cannabinoid receptor 2 (CNR2 gene).
AM156855 - Homo sapiens partial mRNA for cannabinoid receptor 2 (CNR2 gene), allele variant1.
AM156856 - Homo sapiens partial mRNA for cannabinoid receptor 2 (CNR2 gene), allele variant2.
BC095545 - Homo sapiens cannabinoid receptor 2 (macrophage), mRNA (cDNA clone IMAGE:7002009), partial cds.
AB463463 - Synthetic construct DNA, clone: pF1KB7157, Homo sapiens CNR2 gene for cannabinoid receptor 2, without stop codon, in Flexi system.
CU686988 - Synthetic construct Homo sapiens gateway clone IMAGE:100022674 5' read CNR2 mRNA.
JD365613 - Sequence 346637 from Patent EP1572962.
JD346837 - Sequence 327861 from Patent EP1572962.
FJ357036 - Homo sapiens cannabinoid receptor 2 (CNR2) mRNA, exon 1 and 5' UTR.
JD252674 - Sequence 233698 from Patent EP1572962.
JD374041 - Sequence 355065 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P34972 (Reactome details) participates in the following event(s):

R-HSA-419426 Cannabinoid receptors can bind cannabinoids
R-HSA-749454 The Ligand:GPCR:Gi complex dissociates
R-HSA-749456 Liganded Gi-activating GPCRs bind inactive heterotrimeric G-protein Gi
R-HSA-380073 Liganded Gi-activating GPCR acts as a GEF for Gi
R-HSA-373076 Class A/1 (Rhodopsin-like receptors)
R-HSA-418594 G alpha (i) signalling events
R-HSA-500792 GPCR ligand binding
R-HSA-388396 GPCR downstream signalling
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: C6ES44, CB2A, CB2B, CNR2_HUMAN, ENST00000374472.1, ENST00000374472.2, ENST00000374472.3, ENST00000374472.4, NM_001841, P34972, Q4VBK8, Q5JRH7, Q6B0G7, Q6NSY0, uc318lpo.1, uc318lpo.2
UCSC ID: ENST00000374472.5_7
RefSeq Accession: NM_001841.3
Protein: P34972 (aka CNR2_HUMAN or CB2R_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.