Human Gene CHTF18 (ENST00000262315.14_8) from GENCODE V47lift37
  Description: chromosome transmission fidelity factor 18 (from RefSeq NM_022092.3)
Gencode Transcript: ENST00000262315.14_8
Gencode Gene: ENSG00000127586.17_12
Transcript (Including UTRs)
   Position: hg19 chr16:838,620-848,074 Size: 9,455 Total Exon Count: 22 Strand: +
Coding Region
   Position: hg19 chr16:838,685-847,975 Size: 9,291 Coding Exon Count: 22 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:838,620-848,074)mRNA (may differ from genome)Protein (975 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CTF18_HUMAN
DESCRIPTION: RecName: Full=Chromosome transmission fidelity protein 18 homolog; Short=hCTF18; AltName: Full=CHL12;
FUNCTION: Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single- stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18- RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH.
SUBUNIT: Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. During assembly of the CTF18-RFC complex, CTF18 may first assemble into a subcomplex with RFC2, RFC3, RFC4 and RFC5. CTF18 then interacts directly with CTF8, which in turn interacts with DCC1. The CTF18-RFC complex associates with PCNA and with DNA polymerase POLH. The CTF18-RFC complex does not interact with the Rad9/Rad1/Hus1 complex. CTF18 interacts with SMC1A and RAD21.
SUBCELLULAR LOCATION: Nucleus. Note=Associates with chromatin during S phase.
SIMILARITY: Belongs to the activator 1 small subunits family. CTF18 subfamily.
SEQUENCE CAUTION: Sequence=AAH06437.1; Type=Erroneous initiation; Sequence=BAB15766.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 22.84 RPKM in Testis
Total median expression: 246.31 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -25.8065-0.397 Picture PostScript Text
3' UTR -24.1099-0.243 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003593 - AAA+_ATPase
IPR003959 - ATPase_AAA_core

Pfam Domains:
PF00004 - ATPase family associated with various cellular activities (AAA)
PF05496 - Holliday junction DNA helicase RuvB P-loop domain
PF07728 - AAA domain (dynein-related subfamily)

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases
53795 - PEP carboxykinase-like

ModBase Predicted Comparative 3D Structure on Q8WVB6
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details   Gene Details
Gene SorterGene Sorter   Gene Sorter
 RGDEnsembl  SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003677 DNA binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0003689 DNA clamp loader activity
GO:0043142 single-stranded DNA-dependent ATPase activity

Biological Process:
GO:0006260 DNA replication
GO:0007049 cell cycle
GO:1900264 positive regulation of DNA-directed DNA polymerase activity

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0016020 membrane
GO:0031390 Ctf18 RFC-like complex


-  Descriptions from all associated GenBank mRNAs
  LF210743 - JP 2014500723-A/18246: Polycomb-Associated Non-Coding RNAs.
AK128869 - Homo sapiens cDNA FLJ46847 fis, clone UTERU3005264.
AK293846 - Homo sapiens cDNA FLJ56678 complete cds, highly similar to Homo sapiens CTF18, chromosome transmission fidelity factor 18 homolog, mRNA.
AK309804 - Homo sapiens cDNA, FLJ99845.
AK024476 - Homo sapiens mRNA for FLJ00069 protein, partial cds.
BC006437 - Homo sapiens CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:4040321), partial cds.
BC018184 - Homo sapiens CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae), mRNA (cDNA clone MGC:16775 IMAGE:4156341), complete cds.
BC006278 - Homo sapiens CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae), mRNA (cDNA clone MGC:10737 IMAGE:3959495), complete cds.
KJ899421 - Synthetic construct Homo sapiens clone ccsbBroadEn_08815 CHTF18 gene, encodes complete protein.
LF340796 - JP 2014500723-A/148299: Polycomb-Associated Non-Coding RNAs.
LF340794 - JP 2014500723-A/148297: Polycomb-Associated Non-Coding RNAs.
LF340792 - JP 2014500723-A/148295: Polycomb-Associated Non-Coding RNAs.
LF340791 - JP 2014500723-A/148294: Polycomb-Associated Non-Coding RNAs.
LF210741 - JP 2014500723-A/18244: Polycomb-Associated Non-Coding RNAs.
LF340787 - JP 2014500723-A/148290: Polycomb-Associated Non-Coding RNAs.
JD559602 - Sequence 540626 from Patent EP1572962.
JD200875 - Sequence 181899 from Patent EP1572962.
JD140007 - Sequence 121031 from Patent EP1572962.
MA446320 - JP 2018138019-A/18246: Polycomb-Associated Non-Coding RNAs.
MA576373 - JP 2018138019-A/148299: Polycomb-Associated Non-Coding RNAs.
MA576371 - JP 2018138019-A/148297: Polycomb-Associated Non-Coding RNAs.
MA576369 - JP 2018138019-A/148295: Polycomb-Associated Non-Coding RNAs.
MA576368 - JP 2018138019-A/148294: Polycomb-Associated Non-Coding RNAs.
MA446318 - JP 2018138019-A/18244: Polycomb-Associated Non-Coding RNAs.
MA576364 - JP 2018138019-A/148290: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: B7ZBA2, C16orf41, CTF18, CTF18_HUMAN, D3DU68, ENST00000262315.1, ENST00000262315.10, ENST00000262315.11, ENST00000262315.12, ENST00000262315.13, ENST00000262315.2, ENST00000262315.3, ENST00000262315.4, ENST00000262315.5, ENST00000262315.6, ENST00000262315.7, ENST00000262315.8, ENST00000262315.9, NM_022092, Q7Z6Y4, Q7Z6Y6, Q8WVB6, Q9BR83, Q9BRG5, Q9H7K3, uc317gyy.1, uc317gyy.2
UCSC ID: ENST00000262315.14_8
RefSeq Accession: NM_022092.3
Protein: Q8WVB6 (aka CTF18_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.