Human Gene CAMK2B (ENST00000395749.7_8) from GENCODE V47lift37
  Description: calcium/calmodulin dependent protein kinase II beta, transcript variant 1 (from RefSeq NM_001220.5)
Gencode Transcript: ENST00000395749.7_8
Gencode Gene: ENSG00000058404.21_18
Transcript (Including UTRs)
   Position: hg19 chr7:44,256,753-44,365,193 Size: 108,441 Total Exon Count: 24 Strand: -
Coding Region
   Position: hg19 chr7:44,259,661-44,365,020 Size: 105,360 Coding Exon Count: 23 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:44,256,753-44,365,193)mRNA (may differ from genome)Protein (666 aa)
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-  Comments and Description Text from UniProtKB
  ID: KCC2B_HUMAN
DESCRIPTION: RecName: Full=Calcium/calmodulin-dependent protein kinase type II subunit beta; Short=CaM kinase II subunit beta; Short=CaMK-II subunit beta; EC=2.7.11.17;
FUNCTION: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplamic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by Ca(2+)/calmodulin. Binding of calmodulin results in conformational change that relieves intrasteric autoinhibition and allows autophosphorylation of Thr- 287 which turns the kinase in a constitutively active form and confers to the kinase a Ca(2+)-independent activity.
SUBUNIT: CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome (By similarity). Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR.
TISSUE SPECIFICITY: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.
INDUCTION: Activity is induced in skeletal muscle during exercise.
DOMAIN: The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.
PTM: Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=AAC99802.1; Type=Frameshift; Positions=426, 433, 511, 516;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: CAMK2B
Diseases sorted by gene-association score: phencyclidine abuse (10)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 108.20 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 696.31 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -87.90173-0.508 Picture PostScript Text
3' UTR -998.302371-0.421 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020636 - Ca/CaM-dep_Ca-dep_prot_Kinase
IPR013543 - Ca/CaM-dep_prot_kinase-assoc
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase
PF08332 - Calcium/calmodulin dependent protein kinase II association domain
PF13474 - SnoaL-like domain
PF14534 - Domain of unknown function (DUF4440)

SCOP Domains:
56112 - Protein kinase-like (PK-like)
54427 - NTF2-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3BHH - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q13554
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003779 actin binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004683 calmodulin-dependent protein kinase activity
GO:0005088 Ras guanyl-nucleotide exchange factor activity
GO:0005515 protein binding
GO:0005516 calmodulin binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity

Biological Process:
GO:0000165 MAPK cascade
GO:0006468 protein phosphorylation
GO:0007165 signal transduction
GO:0007275 multicellular organism development
GO:0007399 nervous system development
GO:0010976 positive regulation of neuron projection development
GO:0014733 regulation of skeletal muscle adaptation
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0018107 peptidyl-threonine phosphorylation
GO:0030154 cell differentiation
GO:0035556 intracellular signal transduction
GO:0046777 protein autophosphorylation
GO:0048169 regulation of long-term neuronal synaptic plasticity
GO:0051823 regulation of synapse structural plasticity
GO:0051924 regulation of calcium ion transport
GO:0060333 interferon-gamma-mediated signaling pathway
GO:0060998 regulation of dendritic spine development
GO:0061003 positive regulation of dendritic spine morphogenesis
GO:0090129 positive regulation of synapse maturation

Cellular Component:
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005815 microtubule organizing center
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016529 sarcoplasmic reticulum
GO:0030054 cell junction
GO:0030666 endocytic vesicle membrane
GO:0033017 sarcoplasmic reticulum membrane
GO:0043005 neuron projection
GO:0045202 synapse


-  Descriptions from all associated GenBank mRNAs
  AK290148 - Homo sapiens cDNA FLJ75317 complete cds, highly similar to Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II beta (CAMK2B), transcript variant 2, mRNA.
AK294138 - Homo sapiens cDNA FLJ58056 complete cds, highly similar to Calcium/calmodulin-dependent protein kinase type II beta chain (EC 2.7.11.17).
AJ252237 - Homo sapiens mRNA for calcium/calmodulin dependent protein kinase II beta 2 (CaMK II beta 2 gene).
AJ252236 - Homo sapiens mRNA for calcium/calmodulin dependent protein kinase II beta 1 (CaMK II beta 1 gene).
BC019070 - Homo sapiens calcium/calmodulin-dependent protein kinase II beta, mRNA (cDNA clone MGC:29528 IMAGE:5014712), complete cds.
AK222703 - Homo sapiens mRNA for calcium/calmodulin-dependent protein kinase IIB isoform 2 variant, clone: CBR08526.
AF112471 - Homo sapiens calcium/calmodulin-dependent protein kinase II beta subunit mRNA, alternatively spliced, complete cds.
AF112472 - Homo sapiens calcium/calmodulin-dependent protein kinase II beta subunit mRNA, complete cds.
U23460 - Human proline rich calmodulin-dependent protein kinase mRNA, complete cds.
AF078803 - Homo sapiens calcium/calmodulin-dependent protein kinase II beta subunit mRNA, complete cds.
AF081572 - Homo sapiens calcium/calmodulin-dependent protein kinase II beta e' subunit (CAMKB) mRNA, complete cds.
AF081924 - Homo sapiens calcium/calmodulin-dependent protein kinase II beta 6 subunit (CAMKB) mRNA, complete cds.
AF083419 - Homo sapiens calcium/calmodulin-dependent protein kinase II beta e subunit (CAMKB) mRNA, complete cds.
AF140350 - Homo sapiens calcium/calmodulin-dependent protein kinase II beta 7 subunit (CAM2) mRNA, complete cds.
AM392749 - Synthetic construct Homo sapiens clone IMAGE:100002998 for hypothetical protein (CAMK2B gene).
AM393817 - Synthetic construct Homo sapiens clone IMAGE:100002997 for hypothetical protein (CAMK2B gene).
AJ252238 - Homo sapiens mRNA for calcium/calmodulin dependent protein kinase II beta 4 (CaMK II beta 4 gene).
AK315663 - Homo sapiens cDNA, FLJ96755, Homo sapiens calcium/calmodulin-dependent protein kinase (CaMkinase) II beta (CAMK2B), transcript variant 5, mRNA.
KJ905157 - Synthetic construct Homo sapiens clone ccsbBroadEn_14563 CAMK2B gene, encodes complete protein.
KJ905158 - Synthetic construct Homo sapiens clone ccsbBroadEn_14564 CAMK2B gene, encodes complete protein.
AM392792 - Synthetic construct Homo sapiens clone IMAGE:100002747 for hypothetical protein (CAMK2B gene).
AM393562 - Synthetic construct Homo sapiens clone IMAGE:100002748 for hypothetical protein (CAMK2B gene).
AB462968 - Synthetic construct DNA, clone: pF1KB8435, Homo sapiens CAMK2B gene for calcium/calmodulin-dependent protein kinase (CaM kinase) II beta, without stop codon, in Flexi system.
CU676368 - Synthetic construct Homo sapiens gateway clone IMAGE:100019704 5' read CAMK2B mRNA.
AB073352 - Homo sapiens primary neuroblastoma cDNA, clone:Nbla00715, full insert sequence.
AF131776 - Homo sapiens clone 25048 mRNA sequence.
AK091991 - Homo sapiens cDNA FLJ34672 fis, clone LIVER2001164, highly similar to Human proline rich calmodulin-dependent protein kinase mRNA.
AX747297 - Sequence 822 from Patent EP1308459.
JD257879 - Sequence 238903 from Patent EP1572962.
JD232709 - Sequence 213733 from Patent EP1572962.
JD100747 - Sequence 81771 from Patent EP1572962.
U50358 - Human calcium, calmodulin-dependent protein kinase II beta mRNA, partial cds.
JD128981 - Sequence 110005 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q13554 (Reactome details) participates in the following event(s):

R-HSA-442725 Activation of CaMKII
R-HSA-445367 CaMKII enters cytoplasm
R-HSA-444792 CaMKII enters nucleus
R-HSA-416320 Trafficking of GluR1-containing AMPA receptors
R-HSA-5082387 Phosphorylation of HSF1 at Ser230 induces transactivation
R-HSA-443475 Phosphorylation of CREB by CaMKII
R-HSA-442726 Raf activation
R-HSA-432172 Activation of NMDA receptor
R-HSA-432162 Unblocking of NMDA receptor
R-HSA-909552 Phosphorylation of STAT1 at Ser727
R-HSA-442760 Activation of RasGRF
R-HSA-432164 Ca2+ influx into the post-synaptic cell
R-HSA-442732 Ras activation
R-HSA-5672965 RAS GEFs promote RAS nucleotide exchange
R-HSA-442729 CREB phosphorylation through the activation of CaMKII
R-HSA-399719 Trafficking of AMPA receptors
R-HSA-438064 Post NMDA receptor activation events
R-HSA-3371571 HSF1-dependent transactivation
R-HSA-442742 CREB phosphorylation through the activation of Ras
R-HSA-399721 Glutamate binding, activation of AMPA receptors and synaptic plasticity
R-HSA-438066 Unblocking of NMDA receptor, glutamate binding and activation
R-HSA-442755 Activation of NMDA receptor and postsynaptic events
R-HSA-3371556 Cellular response to heat stress
R-HSA-877300 Interferon gamma signaling
R-HSA-112314 Neurotransmitter receptors and postsynaptic signal transmission
R-HSA-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-HSA-2262752 Cellular responses to stress
R-HSA-913531 Interferon Signaling
R-HSA-112315 Transmission across Chemical Synapses
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-112316 Neuronal System
R-HSA-168256 Immune System
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-5683057 MAPK family signaling cascades
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A4D2K0, A4D2K1, A4D2K2, A4D2K3, A4D2K4, A4D2K5, A4D2K6, CAM2, CAMK2, CAMKB, ENST00000395749.1, ENST00000395749.2, ENST00000395749.3, ENST00000395749.4, ENST00000395749.5, ENST00000395749.6, KCC2B_HUMAN, NM_001220, O95437, O95438, O95599, Q13554, Q9UGH7, Q9UGH8, Q9UGH9, Q9UNX0, Q9UNX7, Q9UP00, Q9Y5N4, Q9Y6F4, uc318xri.1, uc318xri.2
UCSC ID: ENST00000395749.7_8
RefSeq Accession: NM_001220.5
Protein: Q13554 (aka KCC2B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.