Human Gene ATP5PF (ENST00000284971.8_9) from GENCODE V47lift37
  Description: ATP synthase peripheral stalk subunit F6, transcript variant 1 (from RefSeq NM_001003703.2)
Gencode Transcript: ENST00000284971.8_9
Gencode Gene: ENSG00000154723.13_12
Transcript (Including UTRs)
   Position: hg19 chr21:27,096,811-27,107,215 Size: 10,405 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg19 chr21:27,096,951-27,102,105 Size: 5,155 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr21:27,096,811-27,107,215)mRNA (may differ from genome)Protein (108 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGeneCardsMGIOMIM
PubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: Q6IB54_HUMAN
DESCRIPTION: RecName: Full=ATP synthase-coupling factor 6, mitochondrial; Short=ATPase subunit F6;
FUNCTION: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain (By similarity).
SUBCELLULAR LOCATION: Mitochondrion. Mitochondrion inner membrane (By similarity).
SIMILARITY: Belongs to the eukaryotic ATPase subunit F6 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 315.19 RPKM in Heart - Left Ventricle
Total median expression: 4745.81 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -13.9059-0.236 Picture PostScript Text
3' UTR -25.40140-0.181 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008387 - ATPase_F0-cplx_f6su_mt
IPR016349 - ATPase_F0-cplx_f6su_mt_subgr

Pfam Domains:
PF05511 - Mitochondrial ATP synthase coupling factor 6

SCOP Domains:
111357 - Mitochondrial ATP synthase coupling factor 6

ModBase Predicted Comparative 3D Structure on Q6IB54
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0015078 hydrogen ion transmembrane transporter activity

Biological Process:
GO:0006811 ion transport
GO:0015986 ATP synthesis coupled proton transport

Cellular Component:
GO:0000276 mitochondrial proton-transporting ATP synthase complex, coupling factor F(o)
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  AL110183 - Homo sapiens mRNA; cDNA DKFZp566A221 (from clone DKFZp566A221).
M73031 - Human H+-ATP synthase coupling factor 6 mRNA, complete cds.
M37104 - Human mitochondrial ATPase coupling factor 6 subunit (ATP5A) mRNA, complete cds.
BC066310 - Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6, mRNA (cDNA clone MGC:87152 IMAGE:4795914), complete cds.
BC001178 - Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6, mRNA (cDNA clone MGC:2243 IMAGE:3357779), complete cds.
AY544129 - Homo sapiens proliferation-inducing protein 36 mRNA, complete cds.
AK311899 - Homo sapiens cDNA, FLJ92165, Homo sapiens ATP synthase, H+ transporting, mitochondrial F0complex, subunit F6 (ATP5J), nuclear gene encoding mitochondrialprotein, mRNA.
KJ890739 - Synthetic construct Homo sapiens clone ccsbBroadEn_00133 ATP5J gene, encodes complete protein.
KJ896477 - Synthetic construct Homo sapiens clone ccsbBroadEn_05871 ATP5J gene, encodes complete protein.
BT007244 - Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 mRNA, complete cds.
CR456950 - Homo sapiens full open reading frame cDNA clone RZPDo834C0516D for gene ATP5J, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6; complete cds, incl. stopcodon.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-7980 - ATP biosynthesis

-  Other Names for This Gene
  Alternate Gene Symbols: ATP5J , ENST00000284971.1, ENST00000284971.2, ENST00000284971.3, ENST00000284971.4, ENST00000284971.5, ENST00000284971.6, ENST00000284971.7, hCG_2007844 , NM_001003703, Q6IB54, Q6IB54_HUMAN, uc317kgk.1, uc317kgk.2
UCSC ID: ENST00000284971.8_9
RefSeq Accession: NM_001003703.2
Protein: Q6IB54

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.