Human Gene ARF6 (ENST00000298316.7_4) from GENCODE V47lift37
  Description: ADP ribosylation factor 6 (from RefSeq NM_001663.4)
Gencode Transcript: ENST00000298316.7_4
Gencode Gene: ENSG00000165527.8_10
Transcript (Including UTRs)
   Position: hg19 chr14:50,359,800-50,363,772 Size: 3,973 Total Exon Count: 2 Strand: +
Coding Region
   Position: hg19 chr14:50,360,455-50,360,982 Size: 528 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:50,359,800-50,363,772)mRNA (may differ from genome)Protein (175 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ARF6_HUMAN
DESCRIPTION: RecName: Full=ADP-ribosylation factor 6;
FUNCTION: GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension.
SUBUNIT: Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24.
INTERACTION: P53365:ARFIP2; NbExp=4; IntAct=EBI-638181, EBI-638194; P21283:ATP6V1C1; NbExp=4; IntAct=EBI-638181, EBI-988663;
SUBCELLULAR LOCATION: Golgi apparatus. Cell membrane; Lipid- anchor. Endosome membrane; Lipid-anchor. Cell membrane. Cell projection, filopodium membrane; Lipid-anchor. Note=Recruited to the cell membrane in association with CYTH2 and ARL4C.
TISSUE SPECIFICITY: Ubiquitous, with higher levels in heart, substantia nigra, and kidney.
SIMILARITY: Belongs to the small GTPase superfamily. Arf family.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: ARF6
Diseases sorted by gene-association score: cholera (26), carrion's disease (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 103.64 RPKM in Esophagus - Mucosa
Total median expression: 1386.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -320.30557-0.575 Picture PostScript Text
3' UTR -648.502790-0.232 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005225 - Small_GTP-bd_dom
IPR024156 - Small_GTPase_ARF
IPR006689 - Small_GTPase_ARF/SAR

Pfam Domains:
PF00025 - ADP-ribosylation factor family
PF00071 - Ras family
PF04670 - Gtr1/RagA G protein conserved region
PF08477 - Ras of Complex, Roc, domain of DAPkinase
PF09439 - Signal recognition particle receptor beta subunit

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1E0S - X-ray MuPIT 2A5D - X-ray MuPIT 2A5F - X-ray MuPIT 2A5G - X-ray MuPIT 2BAO - NMR 2BAU - NMR 2J5X - X-ray MuPIT 2W83 - X-ray 3LVQ - X-ray MuPIT 3LVR - X-ray MuPIT 3N5C - X-ray MuPIT 3PCR - X-ray MuPIT 4FME - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P62330
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0003924 GTPase activity
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0031996 thioesterase binding
GO:0047485 protein N-terminus binding

Biological Process:
GO:0001889 liver development
GO:0007049 cell cycle
GO:0007155 cell adhesion
GO:0007399 nervous system development
GO:0015031 protein transport
GO:0016192 vesicle-mediated transport
GO:0030154 cell differentiation
GO:0030838 positive regulation of actin filament polymerization
GO:0030866 cortical actin cytoskeleton organization
GO:0032456 endocytic recycling
GO:0033028 myeloid cell apoptotic process
GO:0034394 protein localization to cell surface
GO:0035020 regulation of Rac protein signal transduction
GO:0036010 protein localization to endosome
GO:0048261 negative regulation of receptor-mediated endocytosis
GO:0051301 cell division
GO:0051489 regulation of filopodium assembly
GO:0060998 regulation of dendritic spine development
GO:0090162 establishment of epithelial cell polarity
GO:0097178 ruffle assembly
GO:0097284 hepatocyte apoptotic process
GO:1903078 positive regulation of protein localization to plasma membrane
GO:2000009 negative regulation of protein localization to cell surface
GO:2000171 negative regulation of dendrite development

Cellular Component:
GO:0001726 ruffle
GO:0005622 intracellular
GO:0005737 cytoplasm
GO:0005768 endosome
GO:0005769 early endosome
GO:0005794 Golgi apparatus
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005925 focal adhesion
GO:0005938 cell cortex
GO:0010008 endosome membrane
GO:0016020 membrane
GO:0030139 endocytic vesicle
GO:0031527 filopodium membrane
GO:0032154 cleavage furrow
GO:0042995 cell projection
GO:0043209 myelin sheath
GO:0055037 recycling endosome
GO:0055038 recycling endosome membrane
GO:0070062 extracellular exosome
GO:0090543 Flemming body


-  Descriptions from all associated GenBank mRNAs
  AK313790 - Homo sapiens cDNA, FLJ94403, Homo sapiens ADP-ribosylation factor 6 (ARF6), mRNA.
BC008918 - Homo sapiens ADP-ribosylation factor 6, mRNA (cDNA clone MGC:16117 IMAGE:3626100), complete cds.
BC002952 - Homo sapiens ADP-ribosylation factor 6, mRNA (cDNA clone IMAGE:3544168).
M57763 - Human ADP-ribosylation factor (hARF6) mRNA, complete cds.
JD216610 - Sequence 197634 from Patent EP1572962.
JD389995 - Sequence 371019 from Patent EP1572962.
JD129546 - Sequence 110570 from Patent EP1572962.
JD408451 - Sequence 389475 from Patent EP1572962.
JD399266 - Sequence 380290 from Patent EP1572962.
JD177760 - Sequence 158784 from Patent EP1572962.
JD178251 - Sequence 159275 from Patent EP1572962.
JD395677 - Sequence 376701 from Patent EP1572962.
AF047432 - Homo sapiens ADP-ribosylation factor mRNA, complete cds.
JD254021 - Sequence 235045 from Patent EP1572962.
AB463401 - Synthetic construct DNA, clone: pF1KB4062, Homo sapiens ARF6 gene for ADP-ribosylation factor 6, without stop codon, in Flexi system.
CR541964 - Homo sapiens full open reading frame cDNA clone RZPDo834F0734D for gene ARF6, ADP-ribosylation factor 6; complete cds, without stopcodon.
KJ890703 - Synthetic construct Homo sapiens clone ccsbBroadEn_00097 ARF6 gene, encodes complete protein.
AF493885 - Homo sapiens ADP-ribosylation factor protein 6 (ARF6) mRNA, complete cds.
BT019868 - Homo sapiens ADP-ribosylation factor 6 mRNA, complete cds.
CR541939 - Homo sapiens full open reading frame cDNA clone RZPDo834D0734D for gene ARF6, ADP-ribosylation factor 6; complete cds, incl. stopcodon.
DD116323 - Arf6 siRNA inhibiting motility and invasiveness of human cancer cell.
HZ473887 - WO 2016002844-A/1: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
DD116324 - Arf6 siRNA inhibiting motility and invasiveness of human cancer cell.
JD207769 - Sequence 188793 from Patent EP1572962.
JD531123 - Sequence 512147 from Patent EP1572962.
JD109801 - Sequence 90825 from Patent EP1572962.
JD165575 - Sequence 146599 from Patent EP1572962.
BC030291 - Homo sapiens cDNA clone IMAGE:5402602.
JD300766 - Sequence 281790 from Patent EP1572962.
HZ473889 - WO 2016002844-A/3: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
JD260042 - Sequence 241066 from Patent EP1572962.
AL117621 - Homo sapiens mRNA; cDNA DKFZp564M0264 (from clone DKFZp564M0264).
HZ473888 - WO 2016002844-A/2: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.
HZ473890 - WO 2016002844-A/4: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P62330 (Reactome details) participates in the following event(s):

R-HSA-8854293 TBC1D10A (EPI64) binds ARF6:GTP
R-HSA-8854214 TBC/RABGAPs
R-HSA-8875656 MET receptor recycling
R-HSA-9007101 Rab regulation of trafficking
R-HSA-6806834 Signaling by MET
R-HSA-199991 Membrane Trafficking
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-5653656 Vesicle-mediated transport
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: ARF6 , ARF6_HUMAN, ENST00000298316.1, ENST00000298316.2, ENST00000298316.3, ENST00000298316.4, ENST00000298316.5, ENST00000298316.6, NM_001663, P26438, P62330, Q6FGZ2, uc317med.1, uc317med.2
UCSC ID: ENST00000298316.7_4
RefSeq Accession: NM_001663.4
Protein: P62330 (aka ARF6_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.